Tollip与NF-κB在脾肾阳虚型溃疡性结肠炎中的级联表达及温补脾肾法的干预研究

溃疡性结肠炎（UC）多呈慢性反复发作,是结肠癌的癌前病变，迄今尚无特异性治疗方法。中医临床证实其病机演变多为脾肾阳虚，且温补脾肾类方药治疗UC疗效确切，但其证候基础及相关治则治法实验研究尚未取得突破性进展。我们研究发现,以温补脾肾法为指导的久泻灵（理中丸合四神丸）对 UC大鼠结肠黏膜损伤具有修复作用。故基于UC病机规律和前期基础，本研究拟以脾肾阳虚型UC大鼠为研究对象，运用Western blot、免疫组化和RT-PCR技术观察TLR4信号通路下游Tollip和NF-κB关键分子在UC进程中的动态级联表达及相应炎症介质IL-1、IL-6、TNF-α、IFN-γ的对话响应，在此基础上采用温补脾肾方药久泻灵进行干预，进一步从跨膜信号转导和炎症因子串联响应角度揭示温补脾肾法及其方药防治UC的作用机制和药靶部位，以期揭示温补脾肾法治疗UC的科学内涵，为开发防治UC优势中成药的关键技术提供理论支撑。

Ulcerative colitis (UC) is a chronic recurrent diseases, which are characterstic of precancerous lesions of colon cancer, and no specific treatment was found untill now. Evidence of pathogenesis of TCM clinical treatment showed that the key pathological mechanisms of UC is Yang deficiency of spleen and kidney. and the treatment on UC by the law of warming and tonifying kidney and spleen proved effective exactly. but syndrome foundations of molecular basis and relevent experimental study made no breakthrough progress in recent years. Our previous study proved that Jiu Xie Ling granules based on the law of warming and recuperating kidney could cure colonic mucosa injury in UC rats. Based on previous rearch foundation, this study is to explore dynamic expressions of key molecular of TLR4 signal pathway downstream around Tollip and NF-κB in UC and cross talk of corresponding inflammation medias such as IL-1, and IL-6, and TNF-a, and IFN-γof UC by using Western blotting,immunohistochemical and RT-PCR methods, furtherly,Jiu Xie Ling granules based on the law of warming and tonifying kidney is to be used to interve UC to discover treatment mechanisms and drug targets from the point of transmembrane signal transduction and inflammatory factors response, and to reveal the scientific connotation of warming and tonifying kidney and spleen in the treatment of UC, and to support key technology to develope superior Chinese medicines for preventing and treating UC.