甘薯淀粉含量相关基因全长序列单核苷酸多态性分析

甘薯是重要的块根类粮食作物和生物质能源植物，但我国宜种甘薯品种淀粉含量不高，因而开发淀粉含量相关分子标记、鉴定淀粉合成相关调控基因并应用于高淀粉甘薯育种非常重要。前期研究中，申请人利用漯薯8（淀粉含量35.1%）和郑薯20（淀粉含量11.4%）的杂交分离群体，发现甘薯淀粉含量由主效QTL控制。本项目拟以漯薯8和郑薯20及F1代分离群体为材料，通过F1代高淀粉池和低淀粉池的转录组分析，解析甘薯淀粉合成的转录调控特征，鉴定淀粉合成关键调控基因；通过对淀粉含量与淀粉合成相关基因多态性位点(SNPs)的关联分析，筛选淀粉含量相关分子标记，为甘薯分子标记辅助和转基因工程育种提供分子元件。

Sweet potato is an important food crop and bioenginery plant. The starch content of sweet potato widely planted in China is low, therefore it is urgent to mine biomarker associated with starch content and identify regulatory genes orchestrating starch synthesis. In our previous study, we found that starch content is governed by a major QTL using the hybrid separate populations of Luoshu 8 (starch content 35.1%) and Zhengshu 20 (starch content 11.4%) . In this project, we propose to unravel the transcripteomic regulation pattern of starch synthesis and identify key regulatory genes involved in starch synthesis; to mine biomarkers associated with starch content through the association analysis between starch content and the single nucleotide polymorphism of genes relative to starch synthesis. The prospective outcome of this proposal is to provide genetic clue for biomarker-assisted breeding and transgenic manipulation breeding of sweet potato.