Ring和RYBP是高度保守的调控蛋白，Ring参与组蛋白H2A泛素化作用，引起基因沉默和X染色体失活，异常表达导致癌变。RYBP能与Ring通过相同的C端结构域结合，行使转录抑制功能，在发育过程中调控Hox基因的沉默表达。同时GAL4/UAS转基因方法广泛应用于基因的功能研究，为很多领域的研究工作铺平了道路。本研究以鳞翅目昆虫的代表家蚕为研究对象，克隆家蚕Ring和RYBP基因，建立适合家蚕研究的GAL4/UAS转基因系统，结合表达分析、免疫沉淀和酵母双杂交技术等鉴定基因的功能。根据我们前期工作结果和文献报道，采用GAL4/UAS转基因研究方法，建立由A4、FibL和3xP3启动子分别启动的家蚕Ring和RYBP基因的GAL4/UAS转基因超量和干涉表达品系，获得突变表型、个体检测及Hox家族基因的表达分析，阐明家蚕Ring和RYBP在有关组织（器官）发育调控中的生物学功能。

Ring and RYBP are the highly conservative regulation proteins.The Ring mediates the monoubiquitination of histone H2A,causing gene silencing and the X chromosome inactivation. Its abnormal expression leads to cancer.RYBP and Ring can bind together by the same structure of C domain,inhibiting transcription,silencing of Hox genes expression during development.Meanwhile, GAL4/UAS system widely used to the genic function study and facilitated the research in many fields.Silkworm as Lepidoptera insects typifying is our research object.We cloned the BmRing and BmRYBP in silkworm bombyx mori. We established the GAL4/UAS transgenic system which is suitable for functional study in silkworm,combining expression analysis,immune precipitation and yeast two-hybrid system,to identify the function of BmRing and BmRYBP.According to our prophase study and the reported documents,we used the transgenic methods of specific promoter priming GAL4/UAS.We establish the GAL4/UAS transgenic strains that rely on the generation of activator transgenic excessive and interventional expression lines of BmRing and BmRYBP.The GAL4 expression is under control of the A4,FibL and 3xP3 promoters.Obtaining and detecting the mutation phenotype,Hox family gene expression analysis,claritying the biological function of BmRing and BmRYBP in silkworm.