水稻细菌性条斑病抗性新基因的分子标记精细定位与育种利用研究

水稻细菌性条斑病（简称水稻细条病）是水稻的重要病害，已经严重威胁着亚洲热带和亚热带地区水稻生产。由细条病导致的减产一般在15%-20%，严重时达40%-60%。选育抗病品种是防治该病最为经济、有效和环保的途径。水稻细条病抗性被认为是数量性状，主要受多基因控制，因此其基因的定位和克隆较为困难，迄今只有极少的细条病抗性数量性状位点被精细定位，这限制了水稻抗细条病资源在育种中的应用。我们前期从广西普通野生稻中鉴定了2份对水稻细条病具有高抗性的材料，研究表明它们可能携带抗细条病的新的主基因。本项目拟进一步鉴定和精细定位这2份材料所携带的抗性基因，开发实用分子标记，采用常规育种与分子标记辅助选择相结合的方法，把鉴定的抗性基因转育到当前主栽的栽培稻品种中，并且获得一批抗细条病基因导入系、聚合系。研究结果将有助于水稻抗细条病基因的分子标记辅助选择和图位克隆，为培育水稻抗细条病新品种提供理想材料。

Rice bacterial leaf streak (BLS), an important disease in rice, has become a serious threat to rice production in tropical and subtropical regions of Asia. Yield losses from BLS are normally 15% to 20%, but can reach 40% to 60% in severe cases. Breeding resistant varieties is one of the most economic, effective and environmentally safe ways to control the disease. The BLS resistance in rice has proved to be mainly controlled by quantitative trait loci (QTLs). To date, only a few QTLs for BLS resistance have been fine-mapped, which limits the application of the BLS- resistance resources in rice breeding. In our previous studies, 2 rice accessions, which were derived from Guangxi common wild rice (O. rufipogon Griff.), were screened and identified to be highly resistant to BLS. It was shown preliminarily that the BLS resistance in these two accessions might be controlled by a novel gene respectively. In this study, the novel resistance genes will be further verified and fine mapped. The effective markers will be developed to help incorporate these resistance genes into the main rice cultivars. The expected results will facilitate the positional cloning of new resistance genes and provide the ideal materials for the cultivation of BLS- resistance varieties