ITF具有保护和修复肠粘膜的作用，为了探讨ITF发挥这一作用的机制，以及ITF和中药清肠愈疡汤治疗溃疡性结肠炎的作用机理，本项目应用RT-PCR、Western、Elisa、免疫组化等技术，观察注射重组ITF及口服清肠愈疡汤对TNBS大鼠的干预作用。认为ITF能与MUC2结合，促进NF-ΚB的活化，从而降低TGF-β，IL-4，IL-10，升高TNF-α，IL-1β，IL-6的含量，有效降低炎症反应，保护肠粘膜。同时认为，清肠愈疡汤具有清热祛湿，敛疮生肌的功效，能使大鼠粘膜中ITF表达升高，揭示其治疗溃疡性结肠炎的作用机制，证实了溃疡性结肠炎（急性期）湿毒致病的病因说。

ITF has to protect and repair the role of bowel mucosa, in order to explore the mechanism of that role the ITF play, and the ITF and Chinese medicine Qingchang Yuyang Decoction in the treatment of ulcerative colitis mechanism of action, and the project apply RT-PCR, the Western, Elisa, immunohistochemistry, to observe the intervention effect of injection of recombinant ITF and oral Qinchang Yuyang Decoction on TNBS rats. Think that ITF can combine with MUC2, promote NF-ΚB activation, so as to reduce TGF-β, IL-4, IL-10, rise TNF-α, IL-1 β, IL-6 content and reduce inflammation effective, protect the intestinal mucosa. At the same time that, Decoction has the effect of clearing damp-heat in large intestine, Promoting ulcer healing, can make rats in increased expression ITF mucosa, and reveals the treatment of ulcerative colitis mechanism, confirmed the etiology of damp-poison cause in ulcerative colitis (the acute phase).