慢传输型便秘（STC）严重影响现代人的生活质量。ICC数量减少、结构及功能异常致结肠平滑肌收缩减弱、电慢波异常是STC发病的重要机制，C-kit/SCF通路是ICC发生、发育及表型维持的关键。C-kit/SCF下游的PI3K-ATK及PLC-γ通路对于钙离子产生慢波电位至关重要。ICC的表达及下游信号通路的信号传递受损，慢波电位及神经兴奋的产生及传递失去调控可致STC。枳术丸具有健脾运脾，理气导滞通便之功，能促进胃排空和肠推进，改善结肠ICC形态并提高ICC数量及C-kit的表达。我们推测枳术丸可能通过调控C-kit/SCF下游的PI3K/AKT及PLC-γ信号通路来影响Cajal间质细胞的表达达到健脾通便之效。本课题以脾虚证STC小鼠为研究对象，包括在体和体外实验，从形态学、细胞学和分子生物学角度，探讨STC发生的分子机制以及枳术丸的干预作用，从而为该经典方在临床的应用提供新的理论依据。

Slow transit constipation (STC) seriously affects the quality of life of modern people.The decrease in the number of ICC and abnormal structure and function caused by abnormal colon smooth muscle contraction weakened, slow electric wave is one of the important mechanisms on STC , the C-kit/SCF pathway is a key of ICC's occurrence, development and phenotype maintenance. The PI3K/ATK and PLC-γdownstream pathway of C-kit/SCF is vital for calcium slow wave potentials. The expression of ICC and the signal transmission of signaling pathways downstream is damaged, the slow wave potential and nervous excitement of generation and transmission loss of control can cause on STC. Definite pills can invigorating spleen and moving bowels. The single herbs or compatibility of Pill can promote the gastric emptying and intestinal propulsion, improving the ICC colon morphology and increase the number of ICC and the expression of C-kit. So we speculated that the definite technique pill may through regulating the PI3K/ATK and PLC-γdownstream pathway of C-kit/SCF to influence the expression of Cajal interstitial cells and achieve the effect of purging and strong the spleen. This topic proposed by Spleen deficiency syndrome STC mice Including vivo and invitro experiments discuss the molecular mechanism of system on STC generation and definite the intervention effect of intraoperative pill in detail from the perspective of morphology, cytology and molecular biology to open up new areas in the clinical application of the classic party, providing new theoretical basis.