转录因子FOXM1是控制细胞生长的重要分子，其异常表达与包括肝癌在内的多种肿瘤的发生密切相关，但病理生理机制仍有待明晰。通过数据挖掘，我们发现细胞有丝分裂相关基因FOXM1、KIF4A、TPX2在肝癌组织中高表达并与患者的不良预后相关；生物信息学预测KIF4A和TPX2可能被FOXM1转录调控；已获取的实验证据提示KIF4A是FOXM1的直接靶基因；高表达FOXM1和KIF4A能促进肝癌细胞增殖；人肝癌组织中FOXM1、KIF4A和TPX2的表达明显高于癌旁组织。据此我们假设FOXM1通过调控KIF4A和TPX2基因的表达促进肝癌细胞增殖及肿瘤生长。本课题将采用多学科手段在分子、细胞、动物和临床队列等多个层面探讨FOXM1-KIF4A信号轴和FOXM1-TPX2信号轴在肝癌细胞增殖和肿瘤生长中的作用，阐明该信号网络的生物学功能。研究成果将为肝癌的发病机理提供新依据，为肝癌的诊疗提供新靶点。

The transcription factor FOXM1 is an important molecule that controls the growth of cells, and its abnormal expression is closely related to the occurrence of multiple tumors including hepatocellular carcinoma. But the pathophysiological mechanism remains unclear. Through data mining, we found that cell mitotic gene, FOXM1, TPX2 and KIF4A, were highly expressed in liver cancer tissues and correlated with poor survival prognosis of patients. Bioinformatics prediction: KIF4A and TPX2 genes may be regulated by FOXM1 at the transcription level; The relevant experimental evidence obtained suggests that KIF4A is the direct target gene of FOXM1. High expression of FOXM1 and KIF4A can promote the proliferation of hepatocellular carcinoma cells. The expression of TPX2, KIF4A and FOXM1 in human liver cancer tissues was significantly higher than that of adjacent tissues. Therefore, we hypothesized that FOXM1 promotes the proliferation of hepatocellular carcinoma cells and tumor growth by regulating the expression of KIF4A and TPX2 genes. This topic will explore the role of the FOXM1-KIF4A signal axis and FOXM1-TPX2 signal axis in the proliferation and tumor growth of hepatocellular carcinoma cells at the molecular level, cell level, animal level and clinical cohort. And the biological function of the signal network will be clarified. The research results will provide a new basis for the pathogenesis of hepatocellular carcinoma and provide a new target for the diagnosis and treatment of hepatocellular carcinoma.