小胶质细胞活化在阿尔茨海默病（Alzheimer's disease, AD ）发生发展中起重要作用。由β-淀粉样蛋白（β-amyloid, Aβ）沉积激活的小胶质细胞可产生大量生物活性物质，导致神经元损伤，加速AD发展。因此，抑制小胶质细胞异常活化及神经毒性物质释放将成为AD药物干预的靶点。我们既往运用病理形态学、Western Blot、RT-PCR等技术，初步证实了海风藤能抑制Aβ诱导的小胶质细胞激活和炎症反应。但其有效成分和可能机制目前尚不清楚。本课题拟采用活性追踪方法及薄层色谱（TLC）、质谱（MS）、核磁共振谱（NMR）等波谱技术对海风藤进行分离、提纯，并运用RT-PCR及Western Blot等方法初步确定海风藤抑制小胶质细胞异常活化的有效成分及其可能的作用机制，为进一步开发海风藤的临床应用奠定理论基础。

Activation of microglia plays an important role in the occurrence and progression of Alzheimer's disease (AD). Substantial bioactive substances produced by β amyloid protein-activated microglia can lead to neuron damage and promote progression of AD. So we can get a hopothesis that one target for drug intervention of AD is to inhibit abnormal microglia activation and reduce the release of neurotoxins. Our previous results showed that Kadsura Pepper could inhibit microglia activation and inflammatory reaction by pathomorphological, Western Blot, RT-PCR and other methods. However, the active ingredients and the mechanisms are still unknown. To lay a theoretical basis for further clinical application of Kadsura Pepper, we will extract and purify this drug using TLC, MS, NMR, RT-PCR, Western Blot and other methods, and then explore the active ingredient and preliminarily clarify the mechanism of inhibition of microglia activation by it.