灰麝鼩物种复合体的系统演化关系、隐存种与核型多态研究

灰麝鼩(Crocidura attenuata)是广泛分布于我国大陆东南部与海南岛的广布性鼩形目物种，并向西南延伸至整个印支半岛。申请人的先期初步研究表明我国大陆的灰麝鼩实际上是一个物种复合体，包含有以前被认为仅在台湾分布且形态学上无明显差异的台湾灰麝鼩(C. tanakae)和至少一个隐存种。申请人利用分子数据分析的初步结论表明近年在大陆采集的标本中含有台湾灰麝鼩，而依据染色体核型将台湾灰麝鼩确立为独立物种的研究报道Motokawa et al. (2001)因未能认识到灰麝鼩物种复合体的存在而对这两个物种的核型多态现象产生认定错误。申请人拟通过在广大区域采集灰麝鼩复合体标本，利用分子与核型研究方法明确灰麝鼩复合体内的物种系统演化关系、物种分类与分化、潜在隐存种以及古气候地质事件在系统演化中的作用，并将核型多态与分子演化结果进行关联分析，以初步探索核型多态与物种分化的关系。

Asian gray shrew (Crocidura attenuata) is a wide-spread species of Soricomorpha, distributed in Southeast mainland of China and Hainan Island, and its distribution extends to Indo-Chinese Peninsula. Our preliminary studies by molecular and karyotypic data indicate that, in China mainland, Taiwan gray shrew (C. tanakae) is also distributed and taxonomically included in the C. attenuata complex, and this species complex also includes at least one cryptic species. The latest study by Motokawa et al. (2001) raised the Taiwan subspecies C. attenuata tanakae as a distinct species C. tanakae in Taiwan Island by different karyotypes, however, it might be a problem for the multi-karyotypes of these two species because the species complex was not recognized at that time. In this project, we intend to analyze molecular and karyotype data of C. attenuata complex by widely collecting specimens over its distribution areas including the type locality of C. attenuata to study phylogeny, species delimitation, species differentiation, and cryptic species in the C. attenuata complex, associated with the impacts of ancient climate events on historical phylogeny and biogeography. As a preliminary exploration, we will analyze the relationship of multi-karyotype and species differentiation by connecting karyotype data with molecular phylogeny results.