国内外研究证明，血吸虫循环抗原可作为血吸虫病现症感染的重要标志。但由于缺少高灵敏检测循环抗原标志物的方法，导致了临床上血吸虫病人治愈与否难以鉴别。因此，开展高灵敏检测方法的应用基础研究是血吸虫病现症病人诊断急需解决的课题。在近年的研究中，我们发现采用基于金纳米棒光学免疫传感的方法可以显著提高检测的灵敏度。我们拟在此基础上，通过对血吸虫循环抗原的分离纯化，制备高特异性的捕获抗体，借助金纳米棒和静电沉积技术制备金纳米棒光学传感膜。采用化学修饰，分别将巯基与金、羧基与生物分子的氨基共价结合，实现抗体与金纳米棒的连接。循环抗原与抗体结合的金纳米棒因表面结构发生变化而导致光谱位移，借此显示抗原与抗体特异性的分子识别过程，从而创建基于金纳米棒的光学免疫传感检测日本血吸虫循环抗原的方法，以期满足血吸虫病现症感染鉴别诊断的需要，为血吸虫病疫情与传播控制地区低感染度人群传染源的监测奠定基础。

Circulating antigen of Schistosoma japonicum(SjCAg) are excreted by worm living in host. The existence of SjCAg can reflect active infection and worm burden. However，the lack of effective detection method, results the dilemma in identifying whether the schistosomiasis patient is cured. Application of basic research on new method with high sensitivity to detect SjCAg is direly needed. Recently, it appears that the detection sensitivity can be remarkably improved through our gold nanorods(GNRs) optical immune-sensing method. SjCAg can be raised by separation and purification followed by highly special antibody chosed as capture antibody. Gold nanorods(GNRs) were routinely acquired in seed-mediated growth method. The aspect ratios of GNRs can be adjusting with variational amount of AgNO3. The GNRs detection film is developed based on the immobilization of the capture antibody onto GNRs-adsorbed conducting the matrix with free hydroxy group. During the whole progress, Mercaptoundecanoic acid (MUA) facilitated is important and layer-by-layer assembly technology is applied. When the detection film was exposed in the solution with SjCAg, antigen-antibody binding occurred followed by the changes in the optical properties of the films, which were observed in UV-vis spectroscopy. A novel label-free optical immuno-sensing method based on gold nanorods for detecting of SjCAg can be set up,which is helpful to differentiate between the current infection and patient with shistosomiasis after therapy. And also it lays the foundation for monitoring the light infected patients with schistosomiasis in transmission controlled area.