再生水中抗生素在回灌进入地下环境的过程中，其迁移规律、衰减机制及其对土壤微生物的毒性效应尚处于探索阶段。据此，本项目以代表性抗生素-四环素类抗生素为研究对象，研究其在再生水地下回灌和储存过程中基于不同环境条件下的吸附、解吸、降解等过程，揭示抗生素在地下水波动带中的滞留规律、影响机制及其迁移转化过程，进而根据抗生素的衰减规律反演再生水中抗生素最大负荷。研究分析抗生素对土壤微生物的群落结构、生物量、生物活性等影响，进一步阐明抗生素的残留对土壤微生物的毒性效应。本项目以期为再生水进入含水层后的水质劣化预测和其生态风险评价提供一定的理论依据，在保障再生水后续的安全利用和制定相应的治理措施等方面具有一定的应用价值。

In the process of antibiotics in reclaimed water recharge into the underground environment ,its migration law, attenuation mechanism and toxic effects on soil microorganisms are still in the exploratory stage. Accordingly, the project chooses representative antibiotics - tetracycline antibiotics as the object of this study, studies its adsorption, desorption, degradation processes in underground recharge and storage processes of reclaimed water based on different environmental conditions , reveals the retention law ,influence mechanism ,migration and transformation law of antibiotics in the groundwater-fluctuating region, then inverses the maximum load of antibiotics in reclaimed water according to the law of antibiotics attenuation. This study analyses the impact of antibiotics on soil microbial community structure, biomass, and biological activity, and further clarifies the toxic effects of antibiotic residues on soil microorganisms. This project hopes to provide a certain theoretical basis for water quality deterioration prediction and its ecological risk assessment after reclaimed water entering the aquifer, will have application values on safeguarding the following safe use of reclaimed water and developing appropriate treatment measures.