血管吻合术在挽救生命和保全肢体功能的救治过程中起到关键作用。1912年Alexis Carrel 因改进血管缝合技术被授予诺贝尔生理学和医学奖。随后一百年里涌现了包括黏合法、套接法、内置支架法、激光焊接法等多项新技术，但是血管吻合术后的再狭窄和血栓形成问题始终困扰血管吻合术的发展，尤其针于微小血管。美国斯坦福大学医学院应用泊洛沙姆407凝胶结合医用黏合剂的黏合法实现血管的高质量吻合。本课题组前期自主合成了温敏型肝素-泊洛沙姆 (HP) 新材料，HP保留了泊洛沙姆的温敏性能并具有较好的抗凝血活性，将HP制成亲水凝胶，联合医用黏合剂应用于中小血管的吻合，可望改进泊洛沙姆407凝胶的不足，克服吻合口狭窄和栓塞等瓶颈问题。本课题通过温敏性能考察、胶凝温度测定和在体动物血管吻合等一系列实验，充分考察HP亲水凝胶的实用性和安全性，形成原创性成果，解决血管外科手术中血管吻合技术的瓶颈，实现高质量血管吻合

Vascular anastomosis plays a key role in saving life and restoring of limb function. In 1912, Alexis Carrel was awarded the Nobel Prize in Physiology and Medicine due to his contribution in improving vascular closure technology. During following 100 years, many novel techniques developed, such as medical adhesive anastomosis, cuff vascular anastomosis, built-in soluble stent method and laser welding method. However, restenosis and thrombosis after vascular anastomosis always plague the development of vascular anastomosis, especially for small blood vessels. Recently, researchers from Medicine School of Stanford university use poloxamer 407 gel combined with medical adhesive to achieve high-quality vascular anastomosis. Poloxamer is a biocompatible polymer material which has been approved using in vivo by Europe and United States. To improve the application of poloxamer in vascular anastomosis, we have synthesized a new temperature-sensitive material-heparin-Poloxamer (HP). HP not only remains the temperature-sensitive characteristics as Poloxamer, but also enjoys good anti-clotting activity as heparin. In this project, HP hydrophilic gel will be prepared and used in medical adhesive anastomosis. With the advantages of temperature-sensitive characteristics and anti-clotting activity, HP hydrophilic gel is expected to improve the shortcomings of poloxamer 407 gel and overcome the bottlenecks of anastomotic stenosis and thrombosis. With series experiments of temperature-sensitive properties, gelling temperature characteristics and vascular anastomoses in vivo, the practicality and security of HP temperature-sensitive hydrophilic gel will be fully investigated. Through the project, novel techniques will be gained to solve the bottleneck of vascular anastomosis in vascular surgery, improve surgical safety, reduce the difficulty of vascular operation, and achieve high-quality anastomosis.