MicroRNA-210在急性心肌梗死中的作用及其对凋亡-自噬信号通路的影响

目前急性心肌梗死已成为严重威胁人类健康的疾病之一，如何减少心肌细胞在缺血缺氧条件下的凋亡成为现今心肌梗死研究的热点。近期文献报道和我们的研究发现microRNA-210在急性心肌梗死过程和缺血再灌注中具有抗凋亡的作用，有望成为治疗急性心肌梗死的MicroRNA，但其具体作用和机制尚未阐明，本研究目的是探讨microRNA-210在急性心肌梗死中的作用和对凋亡-自噬的影响。通过构建心肌缺氧细胞模型和急性心肌梗死动物模型，利用PCR array、Western Blotting、间接免疫荧光染色、荧光素酶报告基因等方法，研究microRNA-210对凋亡相关蛋白以及自噬相关蛋白转录和表达水平的影响，阐明microRNA-210在急性心肌梗死中的作用和对凋亡-自噬信号通路的影响。本研究将为心肌梗死过程中心肌细胞凋亡、自噬机制提供基础研究证据，对指导临床治疗心肌梗死具有重要意义。

At present, acute myocardial infarction has become one of the diseases that threaten human health. Research about how to reduce the cardiomyocyte apoptosis in myocardial infarction has become very popular. Recent literature reports and our studies indicate that microRNA-210 plays an anti-apoptotic role in acute myocardial infarction and ischemia reperfusion. MicroRNA-210 is expected to be one treatment of acute myocardial infarction. But its specific function and the mechanism have not been clarified. The purpose of this study is to explore the effects of microRNA-210 in acute myocardial infarction and apoptosis-autophagy signal pathway. We will explore the mechanism of microRNA-210 through the construction of myocardial hypoxia model and animal model of acute myocardial infarction, using PCR array, Western Blotting, indirect immunofluorescence staining, luciferase reporter gene method etc. We will study the effects of microRNA-210 on proteins related to apoptosis and autophagy. The study will elucidate the role of microRNA-210 in acute myocardial infarction and effects on apoptosis-autophagy signals pathway. The results of our research will provide basic evidence for cardiomycyte apoptosis and autophagy. It is of great significance to guide the clinical treatment of myocardial infarction.