益气提升法通过LTBP-4上调Fibulin-5表达、影响脱垂性痔组织中弹性纤维活性的研究

在益气提升法治疗脱垂性内痔取得良好临床疗效的基础上，本研究应用分子生物学研究的手段，测定不同脱垂程度的内痔标本中Fibulin-5、LTBP-4和弹性纤维相关ECM和SLRP相关基因的mRNA及蛋白的表达水平，通过研究不同程度脱垂性内痔组织中Fibulin-5、LTBP-4和弹性纤维相关的表达差异，探究Fibulin-5与脱垂性内痔的发生、发展的内在关联性，并进一步通过分析弹性纤维的表达差异、解释潜在的分子生物学机制。同时，本研究应用临床分析研究和分子生物学研究的手段，在补中益气颗粒干扰后的内痔组织成纤维细胞中，通过RRT-PCR，WB和免疫荧光染色等方法，检测Fibulin-5和LTBP-4分别过表达或敲除后，不同组分和不同治疗手段下，相关基因的表达变化，深入研究益气提升法治疗脱垂性内痔的作用

On the basis of ideal clinical efficacy of prolapsed internal hemorrhoids by Tonifying Qi, to test the expressions of mRNA and protein of Fibulin-5、LTBP-4、elastic fiber related ECM、SLRP related genes by applying the research technique of molecular biology and study the expression differences in different degrees of prolapsed internal hemorrhoid samples, to reveal the relationship between the Fibulin-5 and the occurrence and development of prolapsed internal hemorrhoids, then to analysis the elastic fiber related expression differences and explain the possible molecular biology mechanisms in this study. At the same time, to test the related genes’ expression changes in different groups and treatments after the over-expressing or or knockout of Fibulin-5 and LTBP-4 in the fibroblasts intervented by Bu Zhong Yi Qi Granules by use of RT-PCR, WB and Immunofluorescence staining with the help of research methods of clinical analysis and research and molecular biology for disclosing the mechanism of treating prolapsed internal hemorrhoids by Tonifying Qi. The aim of this study is to find the Molecular biological proofs for the theory of distal displacement of the anal cushions, and provide scientific evidences for treating prolapsed internal hemorrhoids.