中国食管癌死亡率居世界首位，探索食管癌有效疗法迫在眉睫。自噬是细胞高度保守的自我更新机制,也是新陈代谢的重要环节和细胞死亡的重要方式。近年研究表明自噬激活可促进肿瘤细胞存活、转移和化疗耐药等生物过程，靶向自噬的抗肿瘤药物研发已成为有效治疗肿瘤的重要策略。对羟基桂皮醛（Hydroxy cinnamaldehyde，CMSP）为本课题组率先从中药木鳖子中提取的具有抗肿瘤活性的化合物，既往研究成果显示其能够诱导食管癌细胞分化。本课题前期研究发现，高浓度CMSP可能通过改变糖代谢途径调节食管癌细胞自噬，诱导凋亡。在此基础上，本项目拟应用免疫荧光、免疫印迹、生物素标记的Pulldown、蛋白质谱、Co-IP等多种分子生物技术探讨CMSP对细胞自噬和凋亡的调节作用，寻找CMSP直接作用靶蛋白，阐明其通过cAMP-PKA-AMPK信号轴调控细胞生物活性的作用机理，为食管癌综合治疗提供候选药物。

The mortality rate of esophageal cancer in China is the highest among the world. It is urgent to explore effective treatment for esophageal cancer. Autophagy is a highly conservative self-renewal mechanism of cells. It is also an important part of metabolism and an important way of cell death. Recent studies have shown that autophagy activation can promote the survival, metastasis and chemoresistance of cancer cells. The research and development of anti-cancer drugs targeting autophagy has become an important strategy for the effective treatment of cancer. Hydroxy cinnamaldehyde (CMSP) is the compound with anti-tumor activity extracted from the fruit of cochinchina momordicae seed, firstly be studied by our team. Previous studies have shown that it can induce the differentiation of esophageal cancer cells. Previous studies have found that high concentration of CMSP may regulate autophagy thus leading to apoptosis of esophageal cancer cells by changing the pathway of glucose metabolism. Based on previous studies, this project intends to explore the function of CMSP on autophagy and apoptosis by immunofluorescence, immunoblotting, biotin-labeled Puldown, protein spectrum, Co-IP and other molecular biotechnologies, thus to find the target protein of CMSP directly interacting, and to elucidate the mechanism of CMSP regulating cell biological activity through the cAMP-PKA-AMPK signal axis, so as to provide drug candidates for comprehensive treatment of esophageal cancer.