乳突( Papilla)是植物叶片表皮细胞下发生的非特异性的细胞抗病组织结构，在抵抗白粉病菌入侵方面起着重要作用，但有关乳突形成及调控抗性的机制尚不十分清楚。前期通过蛋白质差异显示分析，确定了翻译控制瘤蛋白(TCTP)可能参与甜瓜白粉病的应激抗性反应，进一步通过组织细胞学发现，受白粉病菌诱导的野生甜瓜叶片表皮细胞壁更容易产生致密的乳突。本研究拟通过克隆甜瓜TCTP基因，结合转录表达、原生质体定位确定TCTP蛋白的时空表达模式，利用转基因过表达和V1GS瞬时表达鉴定TCTP在抗性乳突结构形成中的关键作用。通过 CRISPR/Cas9基因编辑技术验证TCTP在甜瓜白粉病抗性乳突机制形成中的功能，综合染色质免疫共沉淀、酵母杂交等方法，确定可能参与这一过程的其他关联蛋白。从而建立甜瓜乳突免疫调控的分子网络机制。研究结果将为创制广谱抗性免疫的甜瓜种质材料和瓜类作物白粉病防控提供支撑。

Papilla is a non-specific cellular disease resistant tissue structure under the epidermal cells of plants. It plays an important role in the invasion of powdery mildew（PM）, but the mechanism about formation and resistance regulation is not yet clear. This project will use the protein differential display analysis, determine why TCTP may be involved in stress resistance reaction process of muskmelon, further works found that wild muskmelon leaf epidermal cell wall induced by PM, dense papilla structure is more easy to made. The aim of this study is to clone and analyze melon TCTP gene, and to identify the temporal and spatial expression pattern of TCTP protein by transcriptional expression and protoplast location. We also want to identified the key role of TCTP in the formation of resistant papilla by transgene and VIGS expression. Edit technical validation TCTP in the formation of muskmelon PM resistance mechanism of papilla function through the CRISPR/Cas9. We also use immunoprecipitation method, determination of other genes may be involved in this process, and draw a network about the molecular mechanism of immune regulation of Muskmelon against to PM. The results of the project will provide a new technical for the muskmelon germplasm materials to create broad spectrum resistant PM, and provide theoretical and technical guidance for the improvement of melon varieties.