一株海洋细菌的抗噬菌体突变及对自身代谢的trade-off效应

批准号:
42006093
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
孙越超
依托单位:
学科分类:
生物海洋学与海洋生物资源
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
孙越超
国基评审专家1V1指导 中标率高出同行96.8%
结合最新热点,提供专业选题建议
深度指导申报书撰写,确保创新可行
指导项目中标800+,快速提高中标率
微信扫码咨询
中文摘要
海洋中噬菌体感染是调控微生物群落结构与功能的重要因素,同时,微生物亦进化出各种防御机制以抵抗噬菌体感染,并常伴随着基因突变的发生,然而这些突变可能对细菌自身代谢也产生较大影响,目前却鲜有报道。近期我们获得一株海洋细菌(Roseobacter denitrificans OCh114)的抗噬菌体突变株,发现其基因组中仅糖基转移酶基因产生了突变。本项目拟以宿主该糖基转移酶基因为切入点,通过对该基因进行敲除和回补,明确其在宿主抵抗噬菌体感染中的关键作用;同时研究该突变是否引起宿主基本生理生化特征的改变,并基于组学技术解析其对宿主自身代谢的潜在影响;最后,对野生株和突变株在不同营养共培养条件下的竞争力进行比较。本项目将揭示R. denitrificans Och114突变株抗噬菌体感染的分子基础和宿主抗噬菌体感染的trade-off效应,有助于对海洋中细菌-噬菌体的相互作用有更加全面深入的了解。
英文摘要
Phage infection in the ocean is an important factor regulating the structure and function of microbial communities. Meanwhile, to resist phage infection, the microorganisms have evolved various defense mechanisms to protect themselves. During the anti-phage processes, host genes often mutate and might have a significant impact on their own metabolic activities. Recently, we obtained an anti-phage mutant of the marine bacterium Roseobacter denitrificans Och114, and found that only its glycosyltransferase gene mutated, making the phage unable to absorb to the cell surface of the host. On the basis of our previous work, this project intends to focus on the glycosyltransferase gene, by knocking out and replenishing this gene from host genome, to verify its key role in conferring the anti-phage ability to the host bacterium; Also, we plan to study whether the gene mutation cause changes in the basic physiological and biochemical characteristics of the host bacterium, and analyze its potential effects on host metabolism by using the omics technique; Finally, the growth competitiveness of the wild-type strain and anti-phage mutant strain under different nutritional conditions will be compared. This project will reveal the molecular basis of the bacterial mutant of R. denitrificans OCh114 against phage infection, as well as the trade-off effect of anti-phage mutation on host metabolism, which will contribute to a more comprehensive and in-depth understanding of the interactions between bacteria and phages in the ocean.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
A Novel Method to Create Efficient Phage Cocktails via Use of Phage-Resistant Bacteria
利用噬菌体抗性细菌创建高效噬菌体混合物的新方法
DOI:10.1128/aem.02323-21
发表时间:2022-01
期刊:Applied and Environmental Microbiology
影响因子:4.4
作者:Chengcheng Li;Tongmei Shi;Yuechao Sun;Yongyu Zhang
通讯作者:Yongyu Zhang
DOI:10.21037/jgo-22-965
发表时间:2022-10
期刊:JOURNAL OF GASTROINTESTINAL ONCOLOGY
影响因子:2.1
作者:Lin, Lihong;Zeng, Xiuxiu;Liang, Shanyan;Wang, Yunzhi;Dai, Xiaoyu;Sun, Yuechao;Wu, Zhou
通讯作者:Wu, Zhou
DOI:10.21037/atm-22-4767
发表时间:2022-10
期刊:ANNALS OF TRANSLATIONAL MEDICINE
影响因子:--
作者:Lin, Lihong;Zeng, Xiuxiu;Liang, Shanyan;Wang, Yunzhi;Dai, Xiaoyu;Sun, Yuechao;Wu, Zhou
通讯作者:Wu, Zhou
国内基金
海外基金
