柞蚕放养不但是我国的特色产业，而且是北方山区农民致富的主要产业。目前，柞蚕主产省区保存有124 个品种资源（分属于不同化性、体色和地理系统），由于该产业的分子研究水平较为薄弱，现存品种资源的遗传多样性与系统进化关系仍不清楚，在一定程度上限制了柞蚕品种资源的合理保护和持续利用。为了解决这一柞蚕学科的关键科学问题，本项目拟利用已获得的柞蚕转录组数据开发EST-SSR分子标记。并应用已建立的柞蚕EST-SSR标记筛选技术，获得大量可用的SSR分子标记。最终对现行柞蚕品种的遗传多态性进行鉴定，解析品种资源的遗传多样性和遗传结构，构建基于等位基因频率的品种分化关系。所获结果将为明确柞蚕品种资源的遗传多样性现状、品种的遗传关系，为柞蚕品种资源的合理利用和保护提供依据。

The stocking of Chinese oak silkworm, Antheraea pernyi, is the main industry of peasants in China, especially, in the North mountainous. At present, a total of 124 inbred strain resources, which are divided into different voltinism, skin color, and geosystem, are kept in major producing areas. However, the knowledge on the genetic diversity and evolutionary relationships of these inbred strains remains unclear, due to the lower level of molecular research in this industry. To some extent, it limits the reasonable protection and sustainable utilization of inbred strain resources. To address this key question related to the production of A. pernyi industry, in this project, we will develop EST-SSR molecular markers from the transcriptome data of A. pernyi; and screen plenty of SSRs on the method we had used sucessfully. Ultimately, we will detect the diversity of inbred strain resources; to determine the genetic diversity and genetic structure of these inbred strains; and to reconstruct the strain differentiation relationships. The results obtained in this project will provide the theorical evidences for clarifying the genetic and evolutionary basis of inbred strain resources, and utilization and reservation of these inbred strain resources of A. pernyi.