慢性疲劳综合征(CFS)以持续或反复发作极度疲劳为特征。现代医学无明确、有效的治疗方法和药物，针灸对改善CFS疲劳症状有肯定疗效。申请人前期研究发现，电针可改变CFS大鼠TGF-β通路。但TGF-β发挥作用的具体机制尚不明确。已有研究发现，TGF-β通路可调控炎症反应，我们通过KEGG分析发现，TGF-β通路可以调控NF-κB。在此基础上，我们提出如下假说：通过TGF-β/Smad调控NF-κB通路在电针抗CFS中发挥重要作用。因此，本项目拟通过CFS动物模型，观察电针治疗CFS过程中，TGF-β/Smad的抑制与否，对CFS疗效、认知、行为学改变的影响，以及对NF-κB通路的调控作用；同时，测定炎症因子的变化，以期找到电针治疗CFS的分子机制。本项目的实施，不仅可以阐明电针治疗CFS的分子机制和调控过程，同时还为针灸治疗CFS提供理论基础。

Chronic fatigue syndrome (CFS) is characterized by persistent or recurrent episodes of extreme fatigue. Modern medicine has no clear and effective treatment methods or drugs for CFS. Acupuncture has a positive effect on improving the symptoms of CFS. We had found that electroacupuncture can change the TGF-beta pathway in CFS rats. But the specific mechanism of how TGF-beta plays a role is not clear. It had been reported that TGF-beta can regulate the inflammatory response. Through the KEGG analysis, we found that the TGF-beta can regulate the NF-kappa B. On this basis, we believe that through TGF-beta/Smad regulating the NF-kappa B pathway plays an important role in the treatment of CFS. Therefore, this study intends to describe by CFS model whether TGF-beta/Smad would be restrained or not, the effect, cognition and behavior of electroacupuncture on CFS, and regulative role about NF- kappa B. At the same time, the changes of inflammation related molecules were determined, so as to find the molecular mechanism of electroacupuncture treatment for CFS. The implementation of this project can not only clarify the molecular mechanism and regulation process of electroacupuncture for CFS, but also provide the theoretical base of electroacupuncture for CFS.