Villin-1是一类钙调节的微丝肌动蛋白结合蛋白家族的一员，是一种具有多种功能的细胞骨架结构蛋白。我们的前期研究首次发现与正常人对照相比Villin-1在慢性乙型肝炎患者的肝脏组织中明显升高，并且在hepG2.2.15细胞中敲除Villin-1后，HBV DNA明显降低，提示它参与影响HBV的复制过程。结合细胞骨架蛋白在多种病毒感染疾病中功能，推测Villin-1与HBV编码蛋白结合，辅助病毒在靶细胞中的转运过程，从而影响病毒复制。本项目拟采用一系列分子生物学和细胞生物学方法，筛选出与Villin-1相互结合的HBV编码蛋白并进一步确认其物理性和功能性相互作用，以期阐明Villin-1参与影响乙肝病毒复制的分子机制，为进一步寻找药靶提供新的线索。

Villin-1 belongs to actin-binding family regulated by Ca2+.It is a cytoskeloton protein with multiple functions. We previously found that Villin-1 increased in the liver of chronic hepatitis B as compared with its healthy controls.When Villin-1 was knocked down with siRNA in hepG2.2.15, the HBV DNA titer decreased in the supernatant. Considering the function of cytoskeloten in the virus infection dieases, we speculate that Villin-1 support HBV transportation in host cell by binding to virus protein encoded by HBV. We hope that we can screen out the HBV proteins binding to Villin-1 and confirm the physical and functional interaction in order to clarify the mechanism of Villin-1 in HBV replication.Even we can provide new clues in drug target finding.