前期我们发现验方坎离颗粒在改善心衰患者临床症状同时，还可显著改善心衰鼠血流动力学和耐力型运动能力，改善心肌能量代谢、增加骨骼肌葡萄糖含量、GLUT-4蛋白及基因表达，降低乳酸水平；进而提示通过干预骨骼肌糖代谢对改善心衰时骨骼肌功能受损有益。藉此，本研究以骨骼肌糖代谢为切入点，在体实验以腹主动脉缩窄致心衰模型为研究对象，主要对包括骨骼肌组织中ATP、乳酸、葡萄糖、糖原、LDH及其同功酶，microRNAs/PI3K/PKB/GLUT-4信号途径关键环节指标等进行观察；离体实验以小鼠骨骼肌成肌细胞C2C12为研究对象，观察在血管紧张素II完全阻断、PI3K和PKB部分阻断的糖代谢实验中上述指标的变化；进一步以坎离颗粒干预，研究该药对慢性心衰时骨骼肌糖代谢异常的改善作用及其效应环节。本研究将对阐明中药坎离颗粒干预慢性心衰新的作用机理，丰富和完善中医药治疗慢性心衰的作用机制有重要意义。

Kanli granule has been demonstrated in the previous studies that it could not only improve the symptoms of patients with heart failure, but also ameliorate the hemodynamics, endurance and myocardial energy metabolism, increase glucose contents, GLUT-4 protein and gene expression in skeletal muscle, lower the level of lactic acid in the rats with heart failure. All the improvements have indicated that regulation of glucose metabolism of skeletal muscle may improve the impaired function of skeletal muscle in the heart failure. Therefore, glucose metabolism of skeletal muscle will be the target in the current study using heart failure model induced by aorta coarctation, in which the marks are ATP, lactic acid, glucose, glycogen, LDH and its isoenzyme, microRNAs/PI3K/PKB/GLUT-4 signaing pathway in the skeletal muscle. We will also explore the changes of the above marks when angiotensin II is fully blocked, PI3K and PKB partly blocked respectively using myoblast C2C12 of mouse skeletal muscle. Further study will be explored the intervention of Kanli granule on abnormal glucose metabolism of skeletal muscle during chronic heart failure and the relevant mechanism. The study will elucidate the mechanism of Kanli granule of treating heart failure, thus enrich and perfect TCM theory in this area.