生物防治是国内外植物病害防治的重要手段和发展方向，应用植物体内的有益内生细菌促进植物生长，提高植株的抗病虫能力已成为近一个时期植物病虫害生物防治研究的热点，其中洋葱伯克氏菌群（Bcc）中许多种类或株系已在国外的一些植物病害生物防治中发挥重要作用。本研究以申请人前期从杨树体内筛选获得的对杨树溃疡病等林木病害有高效生防潜能的吡咯伯克霍尔德氏菌Burkholderia pyrrociniaJK-SH007菌株为对象，研究其在杨树体内的内生定殖部位和定殖周期，对杨树等植物的促生抗病效应和稳定性；进行内生细菌促生抗菌物质分离、纯化和化学结构分析，揭示其促生抗病的化学机理；研究其对多品种杨树的接种效应，分析评价其释放于环境后的生态安全性；开展B.pyrrociaJK-SH007全基因组学研究，解析JK-SH007抗病促生功能基因；遗传转化导入其他功能基因，构建具有多种促生抗病抗虫功能的Bcc工程菌株。

In recent years, biological control is an important method and developing drection in controling plant pathogens. Several endophytic bacteria isolated from plant tissues have been reported to be potential biological control agents against phytopathogens and insects and to increase plant growth and development. Several strains of Burkholderia cepacia complex (BCC) have shown effective ability in suppressing many plant diseases. Here, the high-efficient antagonistic bacterium B. pyrrocinia JK-SH007 with biocontrol activity against the poplar canker will be advantaged. Firstly, the colonizing dynamics, disease resistance and growth effect of this strain will be determined. Secondly, isolation, purification and chemical structure of inhibitory and promoting-growth compounds from strain JK-SH007 will be studied to make known chemical mechanism of biocontrol and promoting growth. Thirdly, the inoculation effect of this strain will be determined to evaluate ecological safety of strain JK-SH007. Study on the genome of B. pyrrocinia JK-SH007 will be carried to unravel the biocontrol mechanisms at the molecular level through next generation sequencing technology and bioinformatic analysis. And, the functional analysis of the genes involved in biocontrol or promoting growth will be carried. Finally, the multi-funcation engineered strain will be constructed to improve biocontrol activity of strain JK-SH007 through genetic transformation of other functional genes.