LEC/B3基因是一类种子发育特异、对种子休眠的形成具有正调控功能的转录因子，在拟南芥中已有报道。在水稻中除了OsVP1外一直没有找到其他的同功能基因。甚至，有关水稻胚胎发育异常的突变体也鲜有报道。穗发芽，是禾谷类作物普遍存在的现象。种子休眠的形成机制与穗发芽具有紧密联系。本项目寻找到了一个胚胎发育异常的穗发芽突变体phs116，使用CRISPR-Cas9技术对其进行OsLEC1、OsLEC2等LEC/B3基因的定点敲除，构建phs116/lec1和phs116/lec2双突变体。分析其与lec1、lec2、phs116单突的表型重叠情况，以及LEC/B3基因在phs116中的表达变化，找到它们的网络调控关系。对上述突变体材料和野生型进行RNA-sequencing转录组比较分析。这不仅有助于揭示水稻种子弱休眠和穗发芽的形成机制，还对单子叶植物LEC/B3同功能基因的发现具有重要的意义。

As reported in Arabidopsis thaliana, LEC/B3 gene family was one kind of seed-specific transcriptional factors that positively regulate the seed dormancy. While in rice except for OsVP1, there was no defined homologous gene with the same function as in Arabidopsis thaliana. Actually research on rice embryo-defective mutants were rare to date. Pre-harvest spouting (PHS),which had a highly close relationship with the switch-on of seed dormancy,was a widespread and common issue in crop species. In this project, one rice mutant phs116, which showed serious PHS and displayed great abnormality in its seed development, would be selected as material,upon which the OsLEC/B3 genes, such as OsLEC1 and OsLEC2,would be knockout by CRISPR-CAS9 technique.Thus, the double mutants phs116/lec1and phs116/lec2 would be created. To explore the network of these regulators gene, the over-lapping phenotype among the double mutants and the single mutant Oslec1、Oslec2、phs116 will be investigated, and also, the expression of the OsLEC/B3 genes in phs116 would be measured, compared with those in the wild type. The transcriptomics information among wild-type and those mutants would also be detected and compared through RNA sequencing method. This research would not only play an important role in understanding the underlying mechanism of the initiation of seed dormancy and Pre-harvest spouting in rice, but also shed light on discovering the identical homologous gene of LEC/B3 in monocotyledonous plants.