自然分娩有利于母婴健康，目前产科中常用的机械或药物助产技术均存在不同程度的并发症，寻找更安全、有效的辅助分娩方法仍是当今产科的热点。针灸助产历史悠久，其临床效应已得到初步验证，但尚缺乏系统、明确的机理阐释。前期研究发现，针刺可激活妊娠晚期大鼠子宫肌细胞IP3介导的胞内钙库释放通路。我们推测针刺通过对细胞内/外钙释放/内流通路的调节，上调胞内Ca2+浓度，进而产生促宫缩效应，而针刺助产对子宫肌Ca2+浓度的影响及其信号通路的研究，国内外尚无报道。为证实上述假说，本项目选用经典助产对穴“合谷-三阴交穴”，施以电针干预，通过MTB、分子生物学等检测方法，观察针刺对妊娠晚期大鼠子宫肌Ca2+浓度的影响，系统探讨针刺通过Ca2+途径促进宫缩的助产机制；并揭示针刺通过胞外Ca2+内流途径（L-VGCC通道）影响Ca2+浓度的微观机制。本项目为针刺助产提供科学证据，利于其临床推广，推动生殖健康发展。

Spontaneous labor is beneficial to maternal and child health. At present, machinery or drugs assisted labor technology may cause varies complications, thus finding a more safe and effective method of assisted delivery is a hotspot in obstetrics. Assisting delivery by acupuncture has a long history, and its clinical effect has been initially verified, however, it still lacks the systematic and definitive mechanism to support it. In previous studies, it has been found that acupuncture can activate IP3-dependent Ca2+ release in the uterine muscle cells of late pregnant rats, namely intracellular calcium store release. Therefore we hypothesized that acupuncture increases intracellular Ca2+ concentration to promote uterine contractions, through the intracellular/extracellular calcium release/ current. There is few study report about this. To confirm this hypothesis, this study will choose classic promoting delivery points-- " LI4(Hegu) –SP6(Sanyinjiao) " and electroacupuncture intervention. We will observe the effect of electroacupuncture on myometrial intracellular Ca2+ concentration in late pregnant rats, explore acupuncture mechanisms of promoting uterine contraction by Ca2+ pathway by using the MTB, molecular biology technology and other testing methods, and reveals the microscopic mechanism of acupuncture affect Ca2+ concentration through extracellular Ca2+ influx channel (L-VGCC channel ). This project aims to provide scientific evidence for acupuncture assisted labor, and help for clinical application, which promotes the development of labor health.