隶属于鲤科鱼类的许多物种是我国水产养殖的主要对象。随着我国水产养殖业的发展，养殖密度不断增大, 病毒性等鱼病的发生率也逐渐增加，严重威胁我国水产养殖业的健康发展，培育抗病鱼类新品种是我国水产业发展的重要方向。本项目旨在以鲤科鱼类的斑马鱼和鲫鱼为研究材料，利用CRISPR/Cas9基因编辑、细胞生物学和分子生物学等技术，揭示FOXO基因家族基因：foxo1a、foxo1b、foxo3a、foxo3b和foxo4等在鱼类抗病毒免疫反应中的作用，解析它们参与鲤科鱼类抗病毒天然免疫的基因调控网络，阐明其分子遗传机制。在此基础上，确定用于鲫鱼抗鲤疱疹病毒Ⅱ型CyHV-2感染的候选基因，为培育抗CyHV-2感染的鲫鱼新品种奠定理论和技术基础。

Cyprinid fish are main economical species for aquaculture in China. As development of aquaculture in China, fish diseases, such as virus disease are becoming great threat because of increasing of aquaculture density. To develop species with anti-disease capability is one of the major directions in China aquaculture. In this proposal, we propose to investigate the role of FOXO family, foxo1a, foxo1b, foxo3a, foxo3b and foxo4 in anti-virus response by the techniques including CRISPR/Cas9, cell biology and molecular biology. Moreover, we intend to dissect the gene network that FOXO family is involved in and demonstrate the underlying mechanisms. On the basis of these data, we try to identify the candidate genes for developing new species of crucian carp with capability in anti-CyHV-2 virus infection.