益肾调督法对AD模型大鼠神经元轴突生长的影响及针灸作用机制研究

轴突异常是阿尔茨海默病形成与发展的关键，线粒体不仅是Aβ发挥毒性作用的重要靶位，且线粒体功能及其动力学过程对轴突生长具有十分重要的生物学意义。轴突异常与线粒体损伤有关，且会加重Aβ神经毒性，导致并加速AD的发生与发展。本课题拟在前期益肾调督防治阿尔茨海默病的研究工作基础上，以脑室内注射Aβl-42诱导AD模型大鼠为对象，采用相关分子生物学技术，首次从线粒体损伤诱导轴突异常的角度，探讨线粒体结构与功能的异常及相关蛋白在AD神经元轴突异常过程中的作用机制，分析保护线粒体对轴突生长的影响及其在学习记忆过程中的作用，为深入揭示针灸改善学习记忆功能的作用机制提供全新资料；进一步从对神经元保护角度探讨针灸有效减轻线粒体损伤诱导轴突异常的作用机制，为更好的研究针灸多环节、多靶点的防治AD作用机制提供更多的线索。

Axonal abnormalities is a key to Alzheimer's disease pathology mechanism. The Mitochondria not only is an important new targets to the Aβ toxicit, but also mitochondrial function and its dynamic process has very important significance in biology on axon growth. Axonal abnormalities associated with mitochondrial damage, and will increase the A beta neurotoxicity, cause and accelerate the occurrence and development of AD. Axonal abnormalities is relevant with mitochondrial damage, it will increase the neurotoxicity of Aβ, which cause and accelerate the occurrence and developement of AD. In this paper,based on the previous work, by intraventricular injection of Aβ1-42 induced the AD model rat, using molecular biology techniques, in order to mitochondrial damage induced axonal abnormalities as the breakthrough point, discuss on abnormal mitochondrial structure and function and related protein's regulation to the axonal abnormalities of neuron in AD; Analysis the effection on protecting the mitochondrial to axonal growth and learning and memory;To provide new information on improving the function of learning and memory by acupuncture and moxibustion; Further,from the neuron's protection , we will discuss the mechanism on that acupuncture and moxibustion can lessen the axonal abnormalities induced by mitochondrial's damage, which provide more clues to better investigate,on acu-moxibustion prevent and cure Alzheimer's disease.