Circ_0050102调控miR-1182/NPSR1及MAPK在胰腺癌中的机制研究

胰腺导管腺癌（PC）严重威胁人类健康。环形RNA和微小RNA相互作用并调控多种肿瘤的增殖分化和凋亡，可作为肿瘤标志物及治疗靶点，但尚未在PC中深入研究。前期工作中，我们基于基因芯片结果选出差异表达显著的circ_0050102，通过生信分析和文献查阅提出"circ_0050102调控miR-1182/NPSR1及下游MAPK通路影响PC发生发展"这一假设。预实验中：用QRT-PCR证实了circ_0050102在PC组织中高表达；体外细胞试验发现敲低其表达后PC细胞增殖能力减弱，迁移侵袭受到抑制，细胞周期阻滞及凋亡率明显增加。实验也证实了circ_0050102、miR-1182与NPSR1之间的相互联系。后续研究将测试circ_0050102吸附miR-1182调控NPSR1及下游MAPK通路这一假设，并进一步探索该途径对PC发生发展的影响，最后通过裸鼠成瘤实验在体内进行验证。

Pancreatic ductal adenocarcinoma (PC) is a serious threat to human health. Circular RNA and microRNA interact and regulate the proliferation, differentiation and apoptosis of various tumors. They can be used as tumor markers and therapeutic targets, but they have not been further studied in PC. In the previous work, we selected circ_0050102 for its significant differential expression from microarray analysis. Through bioinformatics analysis and literature review, we proposed the hypothesis that circ_0050102 regulates the miR-1182/NPSR1 pathway and downstream MAPK pathway to affect the development of PC. In the preliminary experiment, qRT-PCR was utilized for detecting the expression of circ_0050102 in PC tumor tissues. CCK-8 assay, cloning formation assay, transwell assay, flow cytometry were employed for in vitro validation of the effects of circ_0050102 on cell proliferation, cell migration and invasion, cell cycle and cell apoptosis in PC. The correlation between circ_0050102, miR-1182 and NPSR1 was also confirmed. Follow-up studies will test the hypothesis that circ_0050102 adsorbs miR-1182 regulates NPSR1 and downstream MAPK pathway, and further explore the effect of this pathway on the development of PC. The hypothesis will be verified in vivo by nude mice tumorigenesis.