Collagen VI在绵羊卫星细胞自我更新及分化中的作用研究

卫星细胞是骨骼肌中位于肌细胞膜和基膜之间的具有增殖分化潜力的肌源性干细胞，其自我更新及分化受多种信号分子及网络的调控。细胞外基质（ECM）对于维持骨骼肌的更新及卫星细胞的增殖意义重大。Collagen VI是骨骼肌ECM的主要成分之一；研究表明Collagen VI在骨骼肌正常功能的维持中起了关键作用。Collagen VI对绵羊卫星细胞自我更新及分化的作用还不清楚。项目拟从绵羊羔羊骨骼肌中分离培养单肌纤维，筛选游离卫星细胞，经单克隆筛选、鉴定后建立细胞系，研究Collagen VI在其自我更新与分化过程的作用，并深入阐明其调节机制。项目拟比较骨骼肌生长有明显差异的绵羊品种间Collagen VI含量及相关细胞信号，研究Collagen VI含量差异是否为导致不同品种绵羊骨骼肌生长速度差异的原因之一。本研究将为提高羊肉生产水平的绵羊育种工作提供科学依据。

Satellite cell is kind of muscle derived stem cell that lies between the sarcolemma of myofibers and the surrounding basal lamina in adult skeletal muscl.Self-renewal and differentiation were regulated by mutiple factors and signaling pathways. Extracellular matrix is necessary for the skeletal muslce functions and satellite cell physiology. One of the main components of ECM is collagen VI. Now, it is clear that collagen VI plays key role in skeletal muscle function maintain. The role of collagen VI in sheep satellite cell renewal and differentiaion is not clear. On current project, single EDL muslce fiber will be isolated from lamb, and satellite cells will be seperated. Using these cells as model, both the role and mechanims of collagen VI during sheep satellite cell renewal and differentiation will be explored. In addition, we will compare the collagen VI contents and related cell signaling in different sheep breeds to verify the correlation between the collagen VI and muscle growth. Our study will provide some provide basic data for the sheep breeding that aims at improvement of meat quality.