医用钛合金表面Ga-Se-Cu抗菌抗肿瘤涂层的制备及性能研究

生物活性植入体需具有极佳的成骨性能、显著的抗肿瘤和抗感染能力，因此对钛合金植入体进行表面改性从而赋予其生物活性非常有意义。鉴于Ga和Se具有抗菌和抗肿瘤特性，Cu具有抗菌和促成骨等生物医学功能，本项目拟采用一步微弧氧化法，将Ga、Se和Cu引入钛合金表面含钙磷盐涂层中，构筑兼具良好生物相容性和抗菌、抗肿瘤活性的Ga-Se-Cu涂层。拟研究电解质组成和浓度、电参数对涂层组成和表面结构特性的影响，明确Ga、Se、Cu单元素进入涂层机制。采用正交设计和统计分析，研究工艺参数影响Ga-Se-C涂层性能的主次顺序，清晰Ga、Se和Cu共同进入涂层机制。分析涂层的Ga、Se和Cu体外释放行为，清晰Ga、Se和Cu共同影响涂层生物相容性、抗菌性和抗肿瘤性的规律，明确Ga、Se和Cu之间对涂层性能的协同或拮抗作用。本项目研究成果可为钛合金植入材料表面多组元多功能涂层构筑提供理论基础和技术支持。

It is very meaningful to titanium alloys for orthopedic implants to equip with excellent biocompatibility and remarkable antibacterial and antitumor activity by modifying their surface with biomimetic coating. Considering the antibacterial and antitumor activitis of gallium (Ga) and selinium (Se) and the antibacterial and osteogenesis activity of copper (Cu), multifunctional Ga-Se-Cu-containing coating with excellent biocompatibility, antibacterial and antitumor activity will be fabricated by one-step micro-arc oxidation (MAO) on titanium alloy surface. The influences of Ga, Se and Cu salt concentrations and electric parameters on the composition, surface morphology and topography, biocompatibility, antibacterial and antitumor activity of the Ga-, Se-, and Cu-containing coating will be studied, and the mechanism for incorportating Ga, Se and Cu into the one-component coatings will be clarified respectively. And then, by using orthogonal design and statistic analysis, the mechanism for incorporating Ga, Se and Cu into the Ga-Se-Cu-containing coating and the influence of processing parameters on the properties of the multi-component coatings will be investigated. Combined with the in vitro release behaviors of Ga, Se and Cu from the Ga-Se-Cu-containing coating, the influencing regularity of Ga, Se and Cu on the biocompatibility, antibacterial and antitumor activity of the coating will be revealed, furthermore, the potential synergistic or antagonistic effect of Ga, Se and Cu on the biological performance of the coating will be explored. These results will provide important theoretical foundation and experimental data for the research and development of multifunctional multi-component coating on medical titanium alloys.