云南撒坝猪是优良的地方品种，是著名的制作云南宣威火腿的原料猪。近年来，致病性大肠杆菌感染所引起的原发性或继发性猪大肠杆菌病仍然是严重威胁我省特色养猪业健康发展极其重要的因素。本项目以云南撒坝猪致病性大肠杆菌为研究对象，运用Real-time PCR、基因缺失突变、流式细胞术、激光共聚焦显微技术、免疫细胞化学等现代分子生物学技术，研究云南撒坝猪致病性大肠杆菌优势血清型菌株与耶尔森强毒力岛(HPI)的分子流行病学特点，探讨云南撒坝猪致病性大肠杆菌HPI基因对撒坝猪多形核白细胞(PMNs)呼吸爆发的影响及其作用的分子机制。以期阐明撒坝猪致病性大肠杆菌HPI的分子流行和进化规律，并从呼吸爆发的角度揭示致病性大肠杆菌感染撒坝猪PMNs过程中毒力因子HPI的分子致病机制，对于丰富并提升致病性大肠杆菌与细胞相互作用的认识水平具有重要的科学意义，为大肠杆菌病的防制提供新的思路。

The Yunnan Saba pig is the excellent local breeding, which is the material of famous Yunnan Xuanwei ham. Recently, the incidental or consequential porcine colibacillosis by pathogenic E. coli infection is still vital factor threaten to the healthy development of swine industry. In this research, the pathogenic E. coli of Yunnan Saba pig will be the object of study, and its dominant serotype and high pathogenic island (HPI)'s molecular epidemiology trait will be studied, and the effects and molecular mechanism of HPI gene on Saba pig poly morphonuclear leukocytes (PMNs)'s respiratory burst will be explored by Real-time PCR, gene deletion and mutation, FCM, Laser confocal microscopy, immunocytochemistry etc. we expect to elucidate the molecular epidemic and evolution regularty of the Yunnan Saba pathogenic E. coli's HPI, and to reveal the molecular pathogenic mechanism of HPI on the Yunnan Saba pig's PMNs infected by Yunnan Saba pathogenic E. coli from respiratory burst angle. The results are very important scientific significance for enriching and promoting the cognitive level between pathogenic E. coli and cells, which can provide the novel approach to prevent and control of colibacillosis.