水稻减数分裂基因P31comet介导DSB形成及联会复合体组装的深度解析

申请人在前期青年基金的资助下，通过图位克隆获得一个水稻减数分裂新基因P31comet，并发现P31comet与另一联会复合体蛋白CRC1互作，进而介导DSB形成和联会复合体组装（PNAS,2016），然而其精确调控水稻减数分裂的分子机制尚不清楚。此外，研究发现P31-CRC1-PAIR1的互作和P31-CRC1-ZEP1的互作分别在重组DSB形成和联会复合体组装中起重要作用。进一步，申请人通过酵母双杂交筛选水稻幼穗文库获得多个候选互作蛋白。本项目在此基础上，拟深入研究P31comet在DSB形成与联会复合体组装的分子机制，挖掘相关互作蛋白并研究其生物学功能，通过转录组和蛋白组学解析其分子调控网络机制，揭示它们协同调控DSB形成与联会复合体组装的分子机制。相关结果将明确P31comet基因在减数分裂中作用，拓宽基因资源，为植物遗传改良提供理论基础和基因资源。

During the implementation of the “National Natural Science Foundation of China Youth Foud(31400169)”, we obtained a novel rice meiosis gene P31comet by map-based cloning. P31comet interacts with another synaptonemal complex protein CRC1, to mediate the DSB formation and the assembly of synaptonemal complex (PNAS, 2016). However, the precise molecular mechanism and protein regulatory network are still unclear. Besides, recent study found that P31comet-CRC1-PAIR1 interaction and P31comet-CRC1-ZEP1 interaction played an important role in DSB formation and synaptonemal complex assembly, respectively. In addition, several candidate interacting proteins were obtained by yeast two hybridization. On this basis, this project will further study the molecular mechanism of p31comet in DSB formation and the assembly of synaptonemal complex, excavate the interacting protein and research its biological function, analyze its molecular regulatory network mechanism through the transcriptome and proteomics methods, and reveal the mechanism of their synergistic regulation of DSB formation and the assembly of synaptonemal complex. The results will clarify the role of p31comet in meiosis, broaden gene resources, and provide theoretical basis and gene resources for plant genetic improvement.