细胞重编程过程中的细胞通讯和命运决定机制研究

细胞重编程过程中存在两种细胞群体：成功进行重编程的细胞和不能进行重编程的细胞，我们的前期研究发现它们之间存在细胞通讯而且对于iPS的产生具有重要的作用，具体的机制还未知。本项目以诱导多能干细胞过程和多能干细胞始发态/原始态转变两个细胞重编程体系为模型，运用前沿的技术手段系统研究细胞群体之间的通讯对表观遗传修饰和细胞代谢的影响，进而参与细胞群体命运转变的调控，揭示细胞通讯对细胞命运转变的基本规律和机制。对体外细胞重编程过程中细胞通讯模式的探究将会促进体内组织和器官形成过程中细胞间相互作用的研究，并且为各种疾病的发生发展提供新的研究视角。

There are two kinds of cell population in the process of somatic reprogramming: reprogrammable and non-reprogrammable cells. Our preliminary research data suggests the communications between this two cell-population play important roles in the generation of iPS, but the detailed mechanisms are unknown. The proposed study will focus on exploring the essential rules and mechanisms of cell communications in two defined cell fate transition: acquired pluripotency of somatic reprogramming and prime-naïve pluripotency transition. We will utilize state-of-the-art molecular biology and biochemistry platform in our lab to investigate how cell communications between cell population change epigenetic modifications and metabolism in cell driving cell fate conversation. The study can provide valuable model to investigate cell communications involved in tissues and organs formation in vivo, furthermore the proposed project could inspire the research of different diseases including initiation and development of cancers with new insights.