胚胎干细胞来源exosomes调控BMSCs的命运防治骨质疏松的作用及机制

骨质疏松是严重威胁老年人群健康的衰老相关性疾病，亟待探索有效防治的新技术。研究提示针对衰老细胞治疗技术可能是有效防治衰老相关性疾病的新策略。研究表明，BMSCs衰老在年龄相关性骨质疏松发生发展过程中起着非常关键的作用。近年研究提示，干细胞来源Exosomes在逆转组织细胞衰老进程、延缓机体衰老等方面可能发挥重要作用。我们前期研究发现胚胎干细胞来源Exosomes（ESC-exos）可拮抗骨质疏松发生与发展，且提示与其运载的多能性因子有关。结合文献报道，我们推测ESC-Exos极可能通过转运Nanog、Oct4等多能性因子，使衰老干细胞重新年轻化而发挥拮抗组织器官衰老的作用。据此，本研究将系统评估ESC-Exos防治骨质疏松及对BMSCs衰老的调控作用，阐明ESC-exos通过转运干性因子调控BMSCs衰老过程防治骨质疏松的分子机制。为利用干细胞治疗衰老相关性疾病提供新思路和新策略。

Osteoporosis is a major age-related disease in threatening the health condition of the elderly, thus, new therapies are urgently needed to prevent and treat this disease. Recent evidence suggests that therapeutic technique targeting senescent cells is probably a new method in treating age-related disease. Cellular aging of BMSCs is proved to play a crucial role in the occurrence and development of osteoporosis. Some striking results have been achieved in treating age-related disease with Stem cell therapy. It is now observed from some studies that stem cell-derived exosome plays a key role in reversing the process of cell aging and delaying body aging. In our previous studies, we found that embryonic stem cell-derived exosomes (ESC-exos) can impede the development of osteoporosis, which is related with the pluripotency factors ESC-exos carry. Combine with the findings of multiple and our previous researches, we speculate that ESC-exos is likely to rejuvenate aging stem cell and restore its normal function by transfer some pluripotency factors (e.g. Nanog, Oct4, Sox2), which hold back the aging progress of tissue and organs. Accordingly, our project aims to systematically evaluate the function and mechanism of ESC-exos in regulating BMSCs aging and clarify the molecular mechanism of ESC-exos transferring pluripotency factors to regulate BMSCs aging in the treatment of osteoporosis, which provides new ideas and new strategies for the use of stem cells in the treatment of aging-related diseases.