番茄WRKY-B蛋白调控侧枝发育的分子机制及少侧枝番茄新种质的创制

在番茄生产的过程中，番茄侧枝是重要的农艺性状之一。阐明番茄侧枝发生的内在机理不仅可以丰富植物侧枝发育的调控机制，通过生物技术手段培育少侧枝的番茄品种还将大大节省番茄生产中的人力财力。在前期研究中，我们明确了WRKY-B正向调控番茄侧枝发育，同时WRKY-B可能通过调控BRC1a、BRC1b等基因的表达来调控侧枝的发育。本申请拟采用功能基因组学的研究思路，深入分析WRKY-B调控番茄侧枝发育的作用机理，为阐明番茄侧枝发育形成的分子机制提供原创性的知识积累；此外，针对番茄生产中整枝打杈耗时耗力、而栽培品种中少侧枝的番茄材料较为匮乏这一现状，我们拟采用CRISPR/Cas9介导的基因编辑技术对WRKY-B基因进行敲除，以期在红果、粉果以及樱桃番茄骨干自交系中创制少侧枝的番茄新种质，为番茄株型育种提供材料积累，为番茄生产提供新策略、新方向。

In the process of tomato production, lateral branching is one of the important agronomic characters in tomato. Elucidation of the internal mechanism of tomato lateral branching can not only enrich the molecular mechanism of tomato WRKY-B regulating lateral branching, but also greatly save the human and financial resources in tomato production with cultivating branchless tomato by the means of biotechnology. Here we showed that WRKY-B positively regulated lateral branching by regulating the expression of BRC1a and BRC1b genes. In this application, we propose to elucidate the action mechanism of WRKY-B in regulating WRKY-B mediated lateral branching, which promises to shed new light on our understanding of lateral branching. In addition, the time-consuming and labor-consuming of pruning and branching in tomato production, and there is no branchless tomato material. We will also employ the CRISPR/Cas9-mediated gene editing system to yield a WRKY-B knock out tomato line and create branchless germplasm in red fruit, pink fruit and cherry tomato inbred lines, which would promise material for breeding new plant type cultivars and provide new strategies and directions for tomato production.