全球气候变暖和生物多样性保护是当今两大研究热点。鸟类的代谢产热特征可以反映其对环境独特的适应机制和进化对策；应激条件下抗氧化防御系统的功能状况在一定程度上反映鸟类对环境的耐受和适应程度。然而，鸟类应对高温环境的代谢产热特征及抗氧化防御机制尚不清楚。本研究以画眉为研究对象，拟在建立体内（整体）和体外（肝细胞）热应激模型的基础上通过测定代谢产热（BMR、甲状腺激素和线粒体呼吸等）；氧化应激（ROS水平、MDA含量和DNA-蛋白质交联等）和抗氧化防御（SOD、CAT及GPX等活力和表达）指标的变化情况，从个体、酶学、细胞、激素和分子水平上揭示：①画眉科鸟类在高温条件下的代谢产热特征和抗氧化防御对策；②画眉科鸟类热应激条件下代谢产热与抗氧化防御之间的相互关系。旨进一步完善鸟类应激生理生态学理论，提高对环境温度升高引发生态变化的预测和监控能力，为全球变暖威胁下鸟类的生物多样性保护提供理论依据。

Global warming and biodiversity conservation are two research hot spots. As one of the most active components of the ecosystem, birds are quite sensitive in response to climate change. The thermoregulation in birds can reflect their unique adaptive mechanism and evolutionary strategy to their environmental conditions. The functional status of antioxidant defense system in birds under stress conditions reflects their tolerance and adaptive capacity to their environment to a great extent. However, the characteristics of metabolic thermogenesis and the mechanism of antioxidant defense in birds under high temperature environment are still unclear. Birds in Timaliidae are not only an important part of China's wildlife resources, but also have a great significance to global biodiversity conservation. In this study, we will detect metabolic thermogenesis (basal metabolic rate, mitochondrial respiration and thyroid hormones); oxidative stress (reactive oxygen species level, malondialdehyde content and DNA-protein crosslinks) and antioxidant defense (the activities and expressions of superoxide dismutase, catalase and glutathione peroxidase ) in hwamei (Garrulax canorus) on the basis of the heat stress model in vivo and in vitro, and explain: (1) the adaptive strategy of the metabolic thermogenesis and antioxidant defense mechanism in birds of Timaliidae under high temperature conditions; (2) the relationship between metabolic thermogenesis and antioxidant defense mechanism under heat stress in birds of Timaliidae based on integrative measurements of organism, enzyme, cell, hormones and molecular. This project will help us to enrich and improve physiological and ecological theory of stress, enhance the ability to predict and monitor ecological changes caused by climate warming, and provide theoretical basis for biodiversity protection in birds.