植物类病变突变体中的局部组织坏死是一种自发形成的程序性细胞死亡，这种遗传上的缺陷往往会导致植物对病原菌的抗性增强和防卫相关基因的组成型表达。然而植物类病变的发生机理复杂多变，目前还难以完全揭示，还有许多与之相关的新基因尚未被分离鉴定出来，而突变体是挖掘新基因、研究基因功能的良好试验材料，本研究以一个扩展型全生育期的水稻类病变突变体lms1（lesion mimic and senilism 1）为材料，对其进行表型鉴定、遗传分析；并应用SSR、Indel等分子标记技术对目标基因LMS1进行精细定位、图位克隆和转基因互补验证；借助分子生物学的方法技术来深入研究基因LMS1的生物学功能，探讨其导致水稻叶片组织类病变坏死的分子机制，以期为揭示植物程序性细胞死亡的调控机制以及植物抗病防卫信号传递途径奠定基础。

Partial cell necrosis in lesion mimic mutants is a type of programmed cell death and this genetic defect often leads to plant disease resistance enhancement and defense genes constitutive expression. But the exact molecular biological mechanism of plant lesion mimic is varied but not well understood, many related new gene has not yet been identified. Mutant is a good test material for new gene clone and gene function analysis. Here we isolated a rice lesion mimic mutant lms1 (lesion mimic and senilism 1) with spreading cell necrosis through all growth stages, and make research about the phenotypic identification, disease resistance testing and genetic analysis. Furthermore, using molecular marker technology including SSR and Indel etc, we try to map-based clone the target gene LMS1 and make transgenic complementary verification. In addition, we would like to find out the biological function of gene LMS1 with the aid of the method of molecular biology technology, and discuss the molecular mechanism of the cause of rice leaf tissue necrosis. We think this research will help to reveal the regulator mechanism of plant programmed cell death and plant resistance defense signal transmission.