GSTs介导的瓜实蝇对阿维菌素代谢抗性分子机制

瓜实蝇是我国广西地区果蔬上的一种重要入侵害虫，施用化学农药阿维菌素是防治该害虫的主要手段之一，农药长期选择和害虫进化导致瓜实蝇产生了抗药性。开展瓜实蝇对阿维菌素抗药性机制研究是对其抗药性治理的重要基础。本项目以选育的瓜实蝇抗阿维菌素和敏感品系为材料，以前期发现的该虫抗阿维菌素相关的谷胱甘肽S-转移酶（GSTs）活性变化及相关mRNA表达为切入点，采用生理生化技术比较敏感和抗性品系GSTs酶活特性差异；应用RNA-Seq和RT-qPCR技术筛选验证抗阿维菌素相关GSTs基因；RACE-PCR技术克隆GSTs抗性相关基因全长和RT-qPCR解析其在不同品系和发育期的表达模式；RNAi干扰和蛋白体外表达技术验证基因功能。项目研究结果将揭示瓜实蝇GSTs介导的代谢抗性分子机制，为瓜实蝇对阿维菌素的抗性治理提供重要的理论基础。

The melon fly Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae) is the most serious pest of fruit and vegetable crops in the Guangxi province of China. At present, abamectin compounds were the main insecticides used to suppress this pest, selection pressure has resulted in resistance. The mechanism research on the insecticide resistance of pest is the basis of insect resistance management (IRM). Knowledge of the underlying molecular mechanisms associated with insecticide resistance is relatively limited in melon fly. In this project, the resistance of detoxication enzyme activities and the dissipation rates of abamectin are comparative analysis among the susceptible strain and resistant to abamectin strain from B. cucurbitae. RT-qPCR, RNAi, expression of recombinant protein and chromatographic analysis will be employed to identify the function of specific expressing genes. The purposes of this project is to explain the metabolic resistance mechanism of melon fly to abamectin. All of this works should be done for further understanding the insect resistance mechanism, and providing theory reference for insect resistance management.