流感病毒极易变异，而批准的抗流感药物仅作用于神经氨酸酶和M2离子通道两个靶点，且耐药问题日益严重。因此，急需探索新的抗流感病毒的药物靶点。M1、NP蛋白高度保守，互作的区域更为保守，NP蛋白因其功能较多，故在NP蛋白上筛选互作区域，探索关键位点可能成为广谱抗流感药物的新靶标。我们前期的工作中筛选出NP蛋白上与M1互作的区域为348-498位氨基酸，尽管此区域尚无NP与M1的相互作用的报道，但有报道可与PB2、PB1蛋白相互作用。因此，本研究拟进一步细化互作区，结合计算机对接及分子动力学法筛选关键位点，从分子生物学角度探讨关键位点的作用机制。此外，还将利用计算机对接等法从本课题组现有的化合物库及商业化数据库 (ZINC) 中，寻找与该位点结合较好的化合物，运用动物模型对筛选出的靶点做进一步的体内验证，为研究和开发新型抗流感药物提供靶点和科学依据。

Influenza virus is extremely easy to variation, approval of anti-flu drug acts only on two targets right now, neuraminidase and M2 ion channel, but resistance is becoming more and more serious. Therefore, it is urgent to explore new drug targets for anti influenza virus. M1, NP protein is highly conserved, and the interaction regions is more conservative, because of more function, NP protein has become the new target of broad-spectrum. In our previous work, the interaction area of NP and M1 protein was screened, the 348-498 amino acids of NP protein, although it has not been reported, but the interaction of PB2 and PB1 proteins has been confirmed. Therefore, this study intends to refine the interaction area, and explore the key sites combined with the computer docking and molecular dynamics method. In the view of molecular biology to explore the mechanism of the key sites, and using animal model to validate the target point after using the computer docking method to screen active compounds in the subject group existing compound library and the commercial database (zinc). From all of these that could provide targets and scientific basis for the research and development of novel anti influenza drugs.