目前研究认为子宫内膜血管生成是子宫内膜容受性建立的重要环节，我们在前期实验中已经证实：针刺在IVF-ET治疗中可以有效的改善子宫内膜容受性，提高受孕率，因此我们提出了VEGF信号通路介导针刺能够改善子宫内膜容受性，进而提高IVF-ET成功率的假说。围绕这一假说拟采用IVF-ET大鼠模型，通过针刺、针刺结合阻断剂等方案，研究针刺对子宫内膜中VEGF信号通路的相关指标的效应表现。利用形态学观察， ELIAS、免疫组化、实时荧光定量PCR、甲基化敏感性高分辨率溶解曲线法（MS-HRM）等实验技术，完成观察针刺干预IVF-ET的效应、阻断VEGF对效应的影响、VEGF或及其受体的基因甲基化与效应的关系的研究，从分子水平阐明针刺通过调节VEGF信号通路改善IVF-ET中超排卵导致子宫内膜容受性不良影响及作用机制，为针灸介入IVF-ET治疗方案提供科学依据。

We find that the Endometrial Blood Vessel Generation is an important part of the establishment of Endometrial Receptivity. We have demonstrated in previous experiments: Acupuncture in IVF-ET treatment can effectively improve Endometrial Receptivity and improve the pregnancy rate, therefore, we propose a VEGF signaling pathway in Acupuncture can improve Endometrial receptivity, thus improving the success rate of IVF-ET Hypothesis. Around the hypothesis intends to adopt the rat model of IVF-ET, through methods of acupuncture, acupuncture combined with blocker, research the related indexes effect performance that acupuncture on the VEGF signaling pathway in endometrial. Using experimental techniques such as Morphological observation, ELIAS, Immunohistochemistry, Real-time quantitative PCR, Methylation-sensitive high resolution melting curve method(MS-HRM), to complete the research of observing the Effect of acupuncture interference IVF-ET, Blocking the impaction VEGF to the effect, the relationship VEGF or gene methylation in its receptors with effect. From the molecular level clarify the adverse effects and mechanism action that acupuncture will improve IVF-ET super ovulation of endometrial receptivity, through adjust VEGF signaling pathway, to provide a scientific basis for acupuncture in IVF-ET treatment.