神经炎症是阿尔茨海默病(AD)重要的病理基础。既往研究表明，“酸甘化阴”经典药对“白芍-甘草”能有效抑制AD神经炎症反应，但两者配伍“相使为用”治疗AD神经炎症的物质基础和作用机制尚不清楚。基于前期对“辛甘化阳”药对配伍的研究基础，本课题拟在整体动物-细胞-分子不同水平，从p38 MAPK/NF-κB信号通路/炎性因子表达评价药对白芍-甘草抗AD神经炎症的协同作用机制；围绕药对-单味药-成分（群）配伍-单体成分，比较白芍-甘草配伍在药效、化学成分上的差异，探讨药对白芍-甘草“酸甘化阴” 配伍的物质基础；结合 LC-MS 串联色谱分析技术和网络药理学方法，以系统生物学新视角，分析药对白芍-甘草治疗AD神经炎症的药效物质-药物效应-效应作用机制之间的关系；阐释白芍-甘草”酸甘化阴“治疗AD神经炎症的协同增效作用机理和物质基础 ，以期为中医药防治AD提供可靠的实验依据，指导临床的合理治疗。

The neuroinflammation is considered to play a critical role in the pathogenesis of Alzheimer’s disease (AD).Previous study showed that herb pairs ofRadix Paeoniae Alba and Glycyrrhizae Radix et Rhizoma(Baishao-Gancao) and its single herb can inhibit neuroinflammation and oxidation in AD. However, its underlying compatibility mechanism and material basis have not been clearly investigated. Based on theory of ‘ Pungent and Swee Flavors Transforming into Yang, Sour and Sweet Flavors Transforming into Yin’ and earlier research results: material Basis and Mechanism of Cassia twig and Liquorice in myocardium ischemia-reperfusion injury (MIRI), this project will study multi-target synergy mechanism of herb pairs of Radix Paeoniae Alba and Glycyrrhizae Radix et Rhizoma(Baishao-Gancao) on expression of p38 MAPK/NF-κB signaling pathway in AD neuroinflammation, from whole animals, tissues, cellular and molecular levels, to represent the theory of ‘Sour and Sweet Flavors Transforming into Yin’ . Secondly, the project will study the active ingredients of Radix Paeoniae Alba and Glycyrrhizae Radix et Rhizoma(Baishao-Gancao) to compare the differences in the effect and chemical composition from different compatibility proportions and extracting methods. And it’s evaluated though Herb Pair compatibily-Single herb-Ingredient(s) compatibility-Monomer compositions as the main line. Thirdly, based on metabolomics and network pharmacology technology, the project will study the relationship among effective ingredients-drug effluent-action mechanism.Finally, from a new perspective,we systematically study the material basis and action mechanism of herb pairs of PRadix Paeoniae Alba and Glycyrrhizae Radix et Rhizoma(Baishao-Gancao) in the improvent effect on neuroinflammation of AD with system biology. We hope to propose some innovative thoughts for the development of TCM treatment of difficulty treated disease such as Alzheimer’s disease and the theory compound compatibility of TCM.