GWAS发掘水稻抗白叶枯病新位点及其功能基因鉴定

利用抗病基因防治水稻白叶枯病是既有效又环保的方法。基于自然群体和由SNP分子标记构建的高密度单倍体图谱的全基因组关联分析GWAS方法能快速高效地定位与特定表型相关联的分子标记及候选基因。在前期研究工作中，我们采用GWAS方法，在水稻第3号染色上定位到1个新的抗白叶枯病基因位点。本项目将在此研究基础上，根据水稻基因组数据选择候选基因，分析候选基因接种白叶枯菌后的表达模式，利用转基因遗传互补鉴定这个新的抗白叶枯病基因，为防治水稻白叶枯病提供新的抗性基因资源，幷为进一步研究水稻自身免疫提供基础。

The utilization of resistance (R) genes is an effective and environmentally friendly way against rice bacterial blight disease, caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Genome-wide association study (GWAS) is a newly developed strategy to map rice genes using a natural population and a high-density haplotype map of the rice genome. Using this technique, we recently mapped one novel bacterial blight R gene locus on rice chromosome 3. In this proposed project, we will predict and select candidate genes at this locus based on the putative function of the genes in the region. The expression pattern of the candidate genes after Xoo infection will be analyzed. Then the function of these candidate genes will be confirmed using transformation and genetic complementary experiments. Taken together， our proposed study holds a promise to identify a novel R gene against Xoo for rice bacterial blight disease control as well as to provide new insights into the rice immunity.