研究表明apelin/APJ系统在肿瘤的血管生成及侵袭转移中起着重要作用。益气养精方前期研究表明该方具有抑制肺癌血管生成及侵袭转移的作用，这与apelin/APJ系统信号通路的相关作用具有相似性，且该方对该通路中相关细胞因子（VEGF、缺氧诱导因子HIF-1α等）及相关信号（PI3K/Akt）有一定的调节作用。本课题拟在前期基础上，动物体内实验用构建表达荧光素酶的肺癌细胞系建立荷瘤鼠模型，采用益气养精方等进行干预，对肿瘤血管生成、侵袭转移性及瘤体中apelin/APJ系统通路及其相关细胞因子进行观察和检测。体外实验观察益气养精方对乏氧微环境下apelin-13促肺癌细胞增殖及侵袭的调节作用以及对HUVEC内皮细胞增殖、管腔形成的影响，并检测益气养精方对apelin/APJ系统通路及其相关细胞因子的作用，以期筛选益气养精方的作用靶点，并从新的角度揭示益气养精、解毒抗癌法治疗肺癌的本质。

Studies show that apelin/APJ signaling pathways plays an important role in tumor angiogenesis, invasion and metastasis. Preliminary studies of Enhance Qi Nourish Jing Formula show that it can inhibit lung cancer angiogenesis, invasion and metastasis. It’s similar to the the role of apelin/APJ and it can also make some adjustments fot apelin/APJ related cytokines in the pathway and the downstream signaling pathways. This study intends to begin on a preliminary basis. In vivo experiments, we’ll establish the tumor-bearing mouse model with the expression of the luciferase of lung cancer cells. After interventing with Enhance Qi Nourish Jing Formula or other drugs, metastases and cytokines in apelin/APJ signal pathway will be observed and tested. In vitro experiments, lung cancer cell lines in hypoxic microenvironment and HUVEC will be treated with Enhance Qi Nourish Jing Formula or other drugs. Then cell proliferation, invasion and cytokines in apelin/APJ signaling pathway will be tested. And lumen formation of HUVEC will also be observed. Thus, to further explore the effects of Enhance Qi Nourish Jing Formula in the apelin/APJ signaling pathway and its associated cytokines, and to evaluate the the role of this formula in inhibiting tumor angiogenesis, invasion and metastasis.