中西药联合用药现已成为临床普遍的用药方式，但其研究目前大多集中在疗效观察、中药对西药单一成分的药代学影响等层面，难以反映中西药联用多组分体内变化与药效变化的相关性特征。 本项目以谷红注射液为示范，正交组合乙酰谷酰胺和红花主要有效部位（黄酮苷类、红花黄色素类和核苷类）组成16个谷红组分配伍方。在构建体外指纹图谱基础上，HPLC/DAD、LC/MSn、同位素标记-PET检测各谷红配伍方静脉给药后在脑缺血动物体内有效成分（乙酰谷酰胺、红花苷、羟基红花黄色素A和鸟苷等）的含量或代谢变化；同步测定各谷红配伍方抗脑缺血损伤的药效学指标（脑功能、血栓与纤溶、自由基氧化、神经递质释放、细胞凋亡等）；基于中西药相互作用原理，引入多种数理分析方法，构建反映中西药多组分特征的药代与药效联合模型，从药剂学、药物代谢、药效学角度阐释谷红多组分相互作用原理和作用机制，为中西药联用的基础研究提供新的技术与方法。

The combined application of Chinese and Western medicines has now become widespread in clinic, but its study mainly focuses on efficacy observation and the effect of Chinese medicine on the pharmacokinetics of single western medical component, which is difficult to reflect the correlational feature of multicomponent drug metabolism in vivo and pharmacodynamics when Chinese and Western medicines are used together. This project chooses Guhong injection as the model prescription and combines acetylglutamine and three effective parts of Flos Carthami (flavonoid glycosides, yellow pigments and nucleosides) according to orthogonal test L16(45). On the basis of the fingerprint in vitro, effective ingredients (acetylglutamine, carthamin, hydroxysafflor yellow A and guanosine) or their metabolic compounds of sixteen different dosage ratio prescriptions of Guhong in cerebral ischemia animal after intravenous administration are detected dynamically by HPLC/DAD, LC/MSn or isotope labeling-PET. The potency indicators such as cerebral function, thrombosis and fibrinolysis, free radical oxidation, neurotransmitter release and neuronal apoptosis are assessed simultaneously. Based on the herb-drug interaction (HDI), the conjunctive models of pharmacokinetics and pharmacodynamics which characterise the multiple components of Chinese and Western medicines are built, and then analyzed by various mathematical analysis method. The interaction and action mechanism among multiple compounds of Guhong are interpreted from the aspects of pharmaceutics, pharmacokinetics and pharmacodynamics, which provides new research technology and method for basic research of the combined application of Chinese and Western medicines.