重组代谢是肿瘤生长的关键，调控肿瘤代谢就意味着控制肿瘤的生长。CD133，又叫prominin-1,作为多种肿瘤干细胞的标记之一，其阳性细胞在肿瘤发生、转移、复发和耐药中起着重要作用。近来发现CD133也是一个重要的、参与细胞功能的膜和胞浆蛋白。我们的前期研究证实CD133可通过影响葡萄糖摄取，自噬反应和ATP合成而调节细胞生存。然而，CD133如何调节能量代谢的详细机制尚不清楚，鉴于CD133在肿瘤干细胞的重要意义，我们提出研究CD133调节能量代谢的课题，拟从明确CD133与葡萄糖转运蛋白的相互作用，比较CD133阳性和阴性细胞包括原代肿瘤细胞和肿瘤细胞系与正常细胞在能量代谢上的不同，及测定CD133对线粒体功能的调节三个方面，来阐明CD133阳性细胞的重组代谢特征，为深入了解肿瘤（干）细胞的代谢特性，揭示潜在肿瘤代谢靶点，做出创新性的贡献。

Reprogramming of tumor metabolism is the key of tumor growth. Regulation and control of tumor metabolism actually are for inhibition of tumor growth. CD133, also called prominin-1 as one of biomarkers for many cancer stem cells, plays an important role in the initiation, metastasis, recurrence and chemoresistance. Recently, CD133 is an important membrane protein participating cell functions. Our preliminary data has identified that CD133 expression influences cell growth and survival basically through regulating glucose uptake, autophagy and ATP synthesis. However, mechanisms of CD133-medited energy metabolism in cancer (stem) cells are elusive. Since CD133 is associated with cancer stem cells, we put forward to this proposal for CD133 regulation of reprogramming of energy metabolism. We plan to investigate the interaction between CD133 and glucose transporters, characterize the metabolisms of CD133 positive or negative primary or tumor cell lines compared with normal cells, and determine how CD133 is involved in the functions of mitochondria. The results will elucidate the metabolic characterization of cancer (stem) cells, which will be the innovative contribution for deeply understanding the metabolism of cancer (stem) cells and unveiling the potential targets for tumor metabolism.