本组前期在广西长寿人群中发现:脂代谢平衡调节基因变异可促进和维护脂代谢平衡,具抗衰老作用。而这些基因变异(G)与环境因素(E)交互作用,G×E交互作用经促进脂代谢平衡延缓人体衰老的效应仍未知;为进一步探究脂代谢与环境交互作用抗衰老机理,本课题假设,脂代谢调节基因(CETP、FOXO、APOE等)变异及其与环境因素交互作用可能与广西长寿人群抗衰老有关。为检验该假设,拟应用NGS、系统发生和生物信息学分析识别及多人群验证上述变异基因;通过检测和比较长寿与当地自然衰老个体上述基因变异、血脂代谢水平和长寿表型特征及相关环境因素差异,获得相关基因变异与长寿关联的生理生化证据,并分析G×E交互作用,从人群，个体和体外的细胞，分子水平检验其促进脂代谢平衡延缓衰老的关键作用机制。明确脂代谢相关基因变异和G×E交互作用对延长寿命贡献，及与机体代谢水平的关系。结果有望为维护老年健康及衰老疾病防治提供新思路。

Our previous studies had found that lipid metabolism regular gene mutations were associated with promoting and maintaining the balance of lipid metabolism, and were linked with anti-aging properties in the Guangxi longevity population. But there is neither report about lipid metabolism regulator genes-environment interaction nor it about the effect of how to promote the balance of lipid metabolism and how to slow the aging process of human being by the GxE. For further analyzing the mechanism of anti-aging effect with genes-environment interaction, we hypothesize that the interaction (G×E) between lipid metabolism gene mutations(G) and environment (E) is one important cause of longevity in the Guangxi population. To test our hypothesis, we will use methods of next-generation sequencing technology, phylogeny tree，bio-informational analysis and validation in many populations to identify lipid metabolism gene mutations associated with longevity. In order to obtain physiological and biochemical evidences of lipid metabolism gene mutations associated with longevity, we will test and compare lipid metabolism gene mutations between longevity population and their local natural aging individuals, as well as their corresponding serum metabolism index levels, phenotype traits of longevity and environmental factors. Then, we will calculate the effect of GxE interaction to determine its impact on human life extension and lipid metabolism. After, we test its mechanism of promoting and maintaining the balance of lipid metabolism and anti-aging properties from crowd, individual and in vitro cell and molecular level. Our study is expected to provide new ideas on how to keep healthy in old people and on prevention and control of age-related diseases.