细胞色素b6/f复合物（Cyt b6/f）是一个多亚基组成的复合体，并作为中间载体驱动线性和环式电子传递。对参与Cyt b6/f各亚基的生物生成和组装调控因子的研究，一直是当前Cyt b6/f复合物研究的热点和难点。前期工作中，我们发现了一个参与PetD组装的关键因子，命名为DAC（Defective Accumulation of Cyt b6/f complex），它与一些未知蛋白共同参与了PetD组装。我们以DAC为诱饵蛋白，成功筛选到了一个与其互作的因子DACI1（DAC Interacted Protein 1）。本项目将进一步对DACI1以及与 DAC共同参与PetD组装的机理进行研究，详细阐明DACI1蛋白的功能，深入挖掘两因子间的相互关系，进而揭示PetD组装调控的机理并解决Cyt b6/f组装中的关键问题。

The cytochrome b6/f complex (Cyt b6/f) is multi-subunit complex and functions in the linear and cyclic electron transport. The current research hotspot and difficulty is to find the regulated factors which involved in the biogenesis and assembly of Cyt b6/f. In previous work, we found a protein DAC (Defective Accumulation of Cyt b6/f complex) that participate in the PetD assembly, and also provided evidence suggesting that it corporate with other unknown proteins to complete this process. We used DAC as a prey and successful screened a new interacted factor DACI1 (DAC Interacted Protein 1). We will further study the function of DACI1 and relationship between DACI1 and DAC in the assembly process of PetD. The identification of these factors and the elucidation of their functions will reveal the mechanism of PetD assembly and solve the key problems during the processes of Cyt b6/f assembly.