全氟化合物作为一类环境有机污染物，严重危害着食品安全、生态环境和人体健康。针对全氟化合物分析，MALDI-TOF-MS质谱技术以其高通量、快速、高灵敏的特点具有巨大应用潜力。但是，适用全氟化合物分析的MALDI基质却非常单一与稀缺。新型MALDI基质的研究成为MALDI-TOF-MS痕量、超痕量分析全氟化合物的关键。因此，本项目拟采用制备介孔羟基磷灰石组装纳米颗粒研究新型MALDI基质，改善与提高MALDI-TOF-MS分析全氟化合物的灵敏度；研究羟基磷灰石对全氟化合物的表面亲和吸附作用，实现复杂样品中全氟化合物的富集分离与定性定量分析。同时，研究全氟化合物在MALDI源正负离子模式下的多种离子化检出方法，提出并阐述新型MALDI基质“电子诱导”、“离子池”、“超软电离”等离子化机理。项目实施将为全氟化合物的MALDI-TOF-MS分析提供新的基质材料与分析方法。

Perfluorinated compounds (PFCs) as environmental organic pollutants have potential hazards to food safety, ecology environment and human health. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technique is considered to be an ideal tool for development of a high-throughput, rapid and high-sensitive analytical approach for trace analysis of PFCs. However, MALDI matrix suitable for PFCs analysis is still very few. Study and development of a novel matrix for trace analysis of PFCs is necessary and significant. Therefore, this study is planned to focus on the development of a novel matrix by preparing porous hydroxyapatite embedded with nanoparticles, which is applicable to MALDI-TOF-MS analysis of PFCs. The detection sensitivity will be improved and enhanced by homogeneous distribution of nanoparticles in the porous of hydroxyapatite. Quantitative analysis of PFCs in real sample will be studied by using the developed MALDI matrix to achieve separation and enrichment of PFCs on the surface of hydroxyapatite with high affinity property. Furthermore, multiple ionization approaches for PFCs under positive and negative ion mode will be investigated and compared as the mechanisms of “electron inducing”, “ion pooling” and “supersoft ionization” are proposed and clarified. The implementation of this study will provide a novel matrix and analytical method for MALDI-TOF-MS analysis of PFCs.