基于微波加热-分子印迹技术的液质联用单次高通量测定人体血清中多种性激素

近年来，液质联用技术在医学检验领域发展迅速成为研究热点，其激素检测几乎可以诊断所有的类固醇相关障碍性疾病，专家预言它必将成为激素检测的核心技术。我们的研究和文献表明，国内外已有液质联用检测性激素进行临床疾病诊断及筛查的报道，但其前处理过程较为复杂耗时且部分激素回收率不高，极大地阻碍了其发展和应用，如何建立快速简便有效的样品前处理方法成为多种性激素检测中迫切需要解决的问题。我们前期采用传统固相萃取-液相色谱法研究了简单样品中的14种性激素，证明采用传统固相萃取法简便可行但部分激素回收率不高，本课题拟在此基础上，提出采用微波加热-分子印迹技术构建对性激素有特异性吸附的磁性碳纳米管接枝分子聚合物提高激素回收率，并建立基于磁性纳米复合材料的在线固相萃取-液质联用检测方法，高通量快速简便自动化的测定多种性激素。这些研究将提高我国性激素检测水平，其原理可用于血清中其他激素及小分子物质的高通量快速检测。

Recently, high performance liquid chromatography–tandem mass spectrometry (HPLC/MS/MS) has been developed quickily and become one of the attractive research in clinical laboratory medicine area. In order to overcome many limitations of immunoassays, HPLC/MS/MS has the potential to become the critical technique in steriod hormones measurement. As is reported, HPLC/MS/MS has been applied for determination of steriod hormones on domestic and overseas clinical laboratory for clinical diagnostics and analysis, however, the pretreatment method is complicated and time-consuming. Thus, a simple, reliable, sensitive and cost-effective method should be developed. In our last research, traditional solid phase extraction(SPE) –HPLC technique has been used for extraction and determination of 14 steriod hormones in simple samples. SPE techquic has been proved to be a simple and effective pretreatment method for steriod hormones measurement, but the recoveries for some hormones are not satisfactory and the time for HPLC determination is long. microwave assisted molecularly imprinting technique is proposed to synthesized magnetic carbon nanotubes molecularly imprinted polymers(MMIPs) to increase selectivity and recoveries of steriod hormones. On the basis of magnetic carbon nanotubes molecularly imprinted polymers, on line SPE-HPLC/MS/MS method was developed for steriod hormones measurement. These studies will improve the level of steriod hormones measurement in our country, and the principle can expand for rapid detection of other hormones and small moleculars in serum.