基于修饰核酸集合配体的细胞膜表面糖组学模式分析的研究

本项目研究目标定位于可用于细胞膜表面糖组学模式分析的修饰核酸集合配体分析系统。利用修饰核酸配体探针与糖组分之间相互作用的强弱性差异，结合Fisher线性判别分析对检测结果进行归类分析，建立修饰核酸集合配体分析系统，实现对不同来源或不同状态细胞膜表面多聚糖的精准区分。期望能够规避多聚糖结构复杂性引起的限制，使得该分析系统实现对细胞膜表面多聚糖信息的原位分析，借此为多聚糖识别分析提供一种可靠的手段，揭示细胞表面多聚糖的识别与细胞类型的相关性，为细胞识别、癌症的早期诊断提供一种精准、快速、有效的检测技术。

The research goal of this project is to focus on building modified nucleic acid ensemble ligands-based analytical system that can be used for pattern analysis of glycomics on cell membrane surface. Based on the different interaction between the modified nucleic acid ligand probes and sugar components and the classification of testing results by Fisher linear discriminant analysis, the modified nucleic acid ensemble ligands-based analytical system was established to accurately distinguish glycan from different sources or different states on the cell membrane surface. To circumvent the limitations arising from the structural complexity of glycan, the analytical system realizes in situ analysis of the glycan information on the membrane, providing a reliable means for the identification and analysis of glycan, and reveals the correlation between cell surface glycan recognition and cell types, providing a precise, rapid and effective detection technique for cell recognition and early diagnosis of cancer.