Zn在前列腺中具有重要作用。前期研究表明Ⅲ型前列腺炎的前列腺液（EPS）中Zn离子浓度降低，血清Zn浓度上升；同时以质谱为基础的iTRAQ- 2DLC-MALDI-TOF-MS蛋白质组技术发现，血清中一组Zn离子相关蛋白在Ⅲ型前列腺异常表达，功能分析显示这些蛋白之间存在相互作用；ELISA、免疫组化及WB发现，这些蛋白在前列腺组织及EPS中表达，且与Zn离子浓度变化呈现不同的变化趋势。提示在前列腺中，Zn离子通过调控多个蛋白复合物，利用不同的信号转导调控模式发挥作用。本研究拟制备Zn离子富集材料，结合基于质谱的Super SILAC定量蛋白质组学新技术，建立解析Zn离子相关蛋白复合物的方法；利用临床EPS样本和动物模型，鉴定Ⅲ型前列腺炎中的Zn相关蛋白调控网络；通过体内外实验进行验证及功能研究，得到具有临床意义的蛋白若干，明确Ⅲ型前列腺炎与Zn离子相关的调控机制，为发展新治疗策略提供依据。

Zinc plays an important role in prostate. Our previous study found that the concentration of zinc with Category Ⅲ prostatitis patients decreases in EPS, and rises in sera. We also found a set of zinc-interacting proteins expressed abnormally in serum of patients with Category Ⅲ prostatitis using iTRAQ-2DLC-MALDI-TOF tandem mass spectrometry. Functional studies show that there are complex interactions among them. Meanwhile, these proteins were also detected in prostatic tissue and EPS by WB, IHC and ELISA. Their expression levels are fluctuant according to the change of Zn concentration, and the trends are different. These results suggest that zinc plays a role by different protein complex with different regulated modules. In this study, we are going to take advantage of zinc enrichment material and a new mass spectrometry-based quantitative proteomics technology named Super SILAC to establish a method to obtain and identify zinc-interacting protein complexes. After that, human EPS samples and animal prostates will be used for getting the zinc-interacting protein network in Category Ⅲ prostatitis. The key proteins will be obtained by the functional studies and validation experiments in vivo and in vitro. These will help clarify the zinc associated pathogenesis of Category Ⅲ prostatitis, and help develop new therapeutic strategies.